Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:224143
Polk RC, et al., The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model. BMC Dev Biol. 2015;15:30
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6406893
|
|
Gene symbol:
|
Pitx2
|
|
Gene name:
|
paired-like homeodomain transcription factor 2
|
|
Probe:
|
Pitx2 cDNA6
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A:
embryonic day 13.5
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4C:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Tbx5tm1.1Jse/Tbx5+
Ts(1716)65Dn/0
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: left atrium |
Weak |
|
4C
|
Expression was much weaker compared with the wild-type.
|
Specimen
S4A:
embryonic day 13.5
(more )
Specimen
S4A:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4B:
embryonic day 13.5
(more )
Specimen
S4B:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4C:
embryonic day 13.5
(more )
Specimen
S4C:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4D:
embryonic day 13.5
(more )
Specimen
S4D:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4I:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Tbx5tm1.1Jse/Tbx5+
Ts(1716)65Dn/0
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4J:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Tbx5tm1.1Jse/Tbx5+
Ts(1716)65Dn/0
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S4K:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Tbx5tm1.1Jse/Tbx5+
Ts(1716)65Dn/0
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
|
S4K
|
Same as in the wild-type.
|
Specimen
S4L:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Tbx5tm1.1Jse/Tbx5+
Ts(1716)65Dn/0
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools