About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:150759 Lallemand Y, et al., Msx genes are important apoptosis effectors downstream of the Shh/Gli3 pathway in the limb. Dev Biol. 2009 Jul 15;331(2):189-98
Assay type: Western blot
MGI Accession ID: MGI:6404246
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Antibody: anti-Gli3 (H-280): sc-20688
Detection system: horseradish peroxidase
Results
Image: 3O ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
Shh-/- E11.5 TS19: forelimb bud Strong (a) involves: 129S1/Sv * 129X1/SvJ Shhtm1Amc/Shhtm1AmcNot Specified Whole limb buds.
Shh-/- Msx1-/- Msx2-/- E11.5 TS19: forelimb bud Strong (b) involves: 129P2/OlaHsd * 129/Sv Msx1tm1Bero/Msx1tm1Bero, Msx2tm1Bero/Msx2tm1Bero, Shhtm1Amc/Shhtm1AmcNot Specified Whole limb buds.
Control E11.5 TS19: forelimb bud Present Not Specified Not Specified Whole limb buds.
Control (Anterior) E11.5 TS19: forelimb bud Strong (c) Not Specified Not Specified Anterior half of limb buds.
Control (Posterior) E11.5 TS19: forelimb bud Ambiguous Not Specified Not Specified Posterior half of limb buds.
Notes:
(a) Expression levels higher than in controls, and similar to levels in anteiror half of normal limb buds.
(b) Expression comparable to that of Shh-/- limb buds.
(c) Expression in anterior half of normal limb buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory