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Gene Expression Data
Assay Details
Assay
Reference: J:150759 Lallemand Y, et al., Msx genes are important apoptosis effectors downstream of the Shh/Gli3 pathway in the limb. Dev Biol. 2009 Jul 15;331(2):189-98
Assay type: Western blot
MGI Accession ID: MGI:6404246
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Antibody: anti-Gli3 (H-280): sc-20688
Detection system: horseradish peroxidase
Results
Image: 3O ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
Shh-/- E11.5 TS19: forelimb bud Strong (a) involves: 129S1/Sv * 129X1/SvJ Shhtm1Amc/Shhtm1AmcNot Specified Whole limb buds.
Shh-/- Msx1-/- Msx2-/- E11.5 TS19: forelimb bud Strong (b) involves: 129P2/OlaHsd * 129/Sv Msx1tm1Bero/Msx1tm1Bero, Msx2tm1Bero/Msx2tm1Bero, Shhtm1Amc/Shhtm1AmcNot Specified Whole limb buds.
Control E11.5 TS19: forelimb bud Present Not Specified Not Specified Whole limb buds.
Control (Anterior) E11.5 TS19: forelimb bud Strong (c) Not Specified Not Specified Anterior half of limb buds.
Control (Posterior) E11.5 TS19: forelimb bud Ambiguous Not Specified Not Specified Posterior half of limb buds.
Notes:
(a) Expression levels higher than in controls, and similar to levels in anteiror half of normal limb buds.
(b) Expression comparable to that of Shh-/- limb buds.
(c) Expression in anterior half of normal limb buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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