About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:273626 Wang K, et al., Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart development. BMC Dev Biol. 2019 Mar 25;19(1):5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6388798
Gene symbol: Insr
Gene name: insulin receptor
Antibody: p-Insulin Rbeta (Tyr 1162/1163): sc-25103
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 2 E11.5 Control: embryonic day 11.5; Igf2tm1Cons/Igf2tm1Cons (more )
Structure Level Pattern Image Note
TS19: ventricle cardiac muscle Present 2_E11.5 control right lower , 2_E11.5 control right upper Much weaker expression.
TS19: ventricle endocardial lining Present 2_E11.5 control right lower , 2_E11.5 control right upper Much weaker expression.
TS19: ascending aorta Present Regionally restricted 2_E11.5 control right upper , 2_E11.5 control right lower Expressed in coronary blood vessel endothelium. Much weaker expression.
Specimen 2 E11.5 Igf2 cKO: embryonic day 11.5; Igf2tm1Cons/Igf2tm1Cons, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: heart ventricle Absent 2_E11.5 cKO right lower , 2_E11.5 cKO right upper
Specimen 2 E12.5 Control: embryonic day 12.5; Igf2tm1Cons/Igf2tm1Cons (more )
Structure Level Pattern Image Note
TS20: ventricle cardiac muscle Present 2_E12.5 control right lower , 2_E12.5 control right upper Much weaker expression.
TS20: ventricle endocardial lining Present 2_E12.5 control right lower , 2_E12.5 control right upper Much weaker expression.
TS20: ascending aorta Present Regionally restricted 2_E12.5 control right lower , 2_E12.5 control right upper Much weaker expression. Expressed in coronary blood vessel endothelium.
Specimen 2 E12.5 Igf2 cKO: embryonic day 12.5; Igf2tm1Cons/Igf2tm1Cons, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: heart ventricle Absent 2_E12.5 cKO right lower , 2_E12.5 cKO right upper
Specimen 2 E14.5 Control: embryonic day 14.5; Igf2tm1Cons/Igf2tm1Cons (more )
Structure Level Pattern Image Note
TS22: ventricle cardiac muscle Present 2_E14.5 control right upper , 2_E14.5 control right lower Much weaker expression.
TS22: ventricle endocardial lining Present 2_E14.5 control right upper , 2_E14.5 control right lower Much weaker expression.
TS22: coronary artery Present Regionally restricted 2_E14.5 control right lower , 2_E14.5 control right upper Much weaker expression. Expressed in coronary blood vessel endothelium.
Specimen 2 E14.5 Igf2 cKO: embryonic day 14.5; Igf2tm1Cons/Igf2tm1Cons, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: heart ventricle Absent 2_E14.5 cKO right upper , 2_E14.5 cKO right lower
Specimen 4 E14.5 Control: embryonic day 14.5; Igf1rtm1Jcbr/Igf1rtm1Jcbr (more )
Structure Level Pattern Image Note
TS22: coronary artery Present Regionally restricted 4_E14.5 control right lower , 4_E14.5 control right upper Much weaker expression. Expressed in coronary blood vessel endothelium.
TS22: ventricle cardiac muscle Present 4_E14.5 control right upper , 4_E14.5 control right lower Much weaker expression.
TS22: ventricle endocardial lining Present 4_E14.5 control right upper , 4_E14.5 control right lower Much weaker expression.
Specimen 4 E14.5 Igf1r cKO: embryonic day 14.5; Igf1rtm1Jcbr/Igf1rtm1Jcbr, Nkx2-5tm1(cre)Rjs/Nkx2-5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: ventricle cardiac muscle Present 4_E14.5 cKO right upper , 4_E14.5 cKO right lower Unchanged expression.
TS22: ventricle endocardial lining Present 4_E14.5 cKO right upper , 4_E14.5 cKO right lower Unchanged expression.
TS22: coronary artery Present Regionally restricted 4_E14.5 cKO right lower , 4_E14.5 cKO right upper Unchanged expression. Expressed in coronary blood vessel endothelium.
Specimen S1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: ventricle cardiac muscle Present S1_right lower , S1_right upper Much weaker expression.
TS20: ventricle endocardial lining Present S1_right lower , S1_right upper Much weaker expression.
TS20: ascending aorta Present Regionally restricted S1_right lower , S1_right upper Much weaker expression. Expressed in coronary blood vessel endothelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory