Probe:	Elmo1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Strong		2A Elmo1 B6
TS25: cerebral cortex	Strong		2A Elmo1 B6
TS25: main olfactory bulb	Strong		2A Elmo1 B6
TS25: pons	Strong		2A Elmo1 B6
TS25: thalamus	Strong		2A Elmo1 B6
TS25: corpus striatum	Moderate		2A Elmo1 B6
TS25: hippocampus	Moderate		2A Elmo1 B6
TS25: medulla oblongata	Moderate		2A Elmo1 B6
TS25: midbrain	Moderate		2A Elmo1 B6
TS25: telencephalon ventricular layer	Moderate		2A Elmo1 B6
TS25: hypothalamus	Weak		2A Elmo1 B6

Structure	Level	Pattern	Image	Note
TS25: brain	Present	Regionally restricted	2A Elmo1 rl	Authors report overall expression not severely affect by the mutation.
TS25: cerebral cortex	Present	Regionally restricted	2A Elmo1 rl	Authors report layer-dependent expression seemed affected at E17.

Structure	Level	Pattern	Image	Note
TS25: brain	Strong	Regionally restricted	2B Reelin Elmo1	Authors report high expression in superficial regions of the brain with intense Reln expression.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		3A Elmo1
TS28: cerebral cortex	Strong		3A Elmo1
TS28: main olfactory bulb	Strong		3A Elmo1
TS28: pontine nucleus	Strong		3A Elmo1
TS28: thalamus	Strong		3A Elmo1
TS28: hippocampus	Strong		3A Elmo1

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present		3B Elmo1 (upper)
TS28: frontal cortex	Present		3B Elmo1 (upper)
TS28: main olfactory bulb	Present		3B Elmo1 (upper)
TS28: somatic motor system	Present		3B Elmo1 (upper)

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		3B Elmo1 (middle)
TS28: corpus striatum	Present		3B Elmo1 (middle)
TS28: orbital area	Present		3B Elmo1 (middle)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		3B Elmo1 (lower)
TS28: medulla oblongata	Present		3B Elmo1 (lower)
TS28: paraflocculus	Present		3B Elmo1 (lower)

Structure	Level	Pattern	Image	Note
TS28: medial dorsal thalamic nucleus	Strong		3C Elmo1 (left)
TS28: superior colliculus	Strong		3C Elmo1 (left)
TS28: cerebral cortex	Present	Regionally restricted	3C Elmo1 (left)	Moderately high expression at boundary between cortical layer VI and underlying white matter, and in cells of laver IV.
TS28: hippocampus	Strong		3C Elmo1 (left)

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Present		3C Elmo1 (right)

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Present		4A Elmo1 WT	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.
TS28: caudate-putamen	Present		4A Elmo1 WT	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.
TS28: globus pallidus	Present		4A Elmo1 WT	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	4A Elmo1 rl

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	4C WT

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	4C rl	Authors report different cellular expression patterns compared with that of B6 brains.

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Moderate	Regionally restricted	5A Elmo1 (1st row)	Moderately high expression in cells around lover layers IV-VI.
TS28: infralimbic area	Strong	Regionally restricted	5A Elmo1 (1st row)

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Present		5A Elmo1 (2nd row)	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.
TS28: caudate-putamen	Present		5A Elmo1 (2nd row)	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.
TS28: globus pallidus	Present		5A Elmo1 (2nd row)	Expression in caudate putamen, nucleus accumbens and globus pallidus slightly higher than that of Elmo2 and Elmo3.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		5A Elmo1 (3rd row)
TS28: neocortex layer	Moderate	Regionally restricted	5A Elmo1 (3rd row)	Moderately high expression in cells around lover layers IV-VI.
TS28: amygdala	Present		5A Elmo1 (3rd row)

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		5A Elmo1 (4th row)	High expression in piriform cortex in vicinity of intermediate-high expression in cells of lateral olfactory tract and olfactory tubercle.
TS28: lateral habenular nucleus	Weak		5A Elmo1 (4th row)
TS28: dorsomedial hypothalamic nucleus	Present		5A Elmo1 (4th row)

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5B Elmo1	Authors report regional expression patterns roughly similar to those in B6 mouse brains.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		6A Reelin Elmo1
TS25: thalamus	Present		6A Reelin Elmo1

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		6A Elmo1 Elmo2
TS25: thalamus	Present		6A Elmo1 Elmo2

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		6A Elmo1 Elmo3
TS25: thalamus	Present		6A Elmo1 Elmo3

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Moderate	Regionally restricted	6B Elmo1	Moderately high expression at boundary between cortical layer VI and underlying white matter, and in cells of laver IV.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		7A Elmo1

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7B Elmo1 B6

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Ambiguous		7B Elmo1 rl

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7C Elmo1 B6

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Ambiguous		7C Elmo1 rl

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		8A Elmo1
TS28: hippocampus	Present		8A Elmo1

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		8A Elmo1 GAD
TS28: dentate gyrus	Present	Scattered	8A Elmo1 GAD	Authors report expression in some Gad1-expressing interneurons scattered in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		9A Elmo1 P7

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		9A Elmo1 P21

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Scattered	9B Elmo1

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Regionally restricted	9C Elmo1 B6	Expressing cells around Purkinje cell plate and deep cerebellar nucleus.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Regionally restricted	9C Elmo1 rl	Expressing cells around Purkinje cell plate and deep cerebellar nucleus abberrantly localized in reeler cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		10A Elmo1 P7	Expression localized predominantly in inner external granular layer, Purkinje cell layer, and intermediately in internal granular cells layer.
TS28: cerebellum external granule cell layer	Present		10A Elmo1 P7	Expression localized predominantly in inner external granular layer, Purkinje cell layer, and intermediately in internal granular cells layer.
TS28: cerebellum internal granule cell layer	Present		10A Elmo1 P7	Expression localized predominantly in inner external granular layer, Purkinje cell layer, and intermediately in internal granular cells layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		10A Elmo1 P21	Expression in Purkinje cell layers. Expression localized in cells of molecular layer and a few large cells of the internal granular layer.
TS28: cerebellum internal granule cell layer	Present		10A Elmo1 P21	Expression in Purkinje cell layers. Expression localized in cells of molecular layer and a few large cells of the internal granular layer.
TS28: cerebellum molecular layer	Present		10A Elmo1 P21	Expression in Purkinje cell layers. Expression localized in cells of molecular layer and a few large cells of the internal granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	10B Elmo1 GAD	Expression in Gad1-expressing cerebellar neurons, namely in Purkinje cells of Purkinje cell layer, stellate cells and basket cells of molecular layer, and Golgi cells of internal granular layer.
TS28: cerebellum internal granule cell layer	Present	Regionally restricted	10B Elmo1 GAD	Expression in Gad1-expressing cerebellar neurons, namely in Purkinje cells of Purkinje cell layer, stellate cells and basket cells of molecular layer, and Golgi cells of internal granular layer.
TS28: cerebellum molecular layer	Present	Regionally restricted	10B Elmo1 GAD	Expression in Gad1-expressing cerebellar neurons, namely in Purkinje cells of Purkinje cell layer, stellate cells and basket cells of molecular layer, and Golgi cells of internal granular layer.

Structure	Level	Pattern	Image	Note
TS25: neocortex	Strong
TS25: inferior colliculus	Present
TS25: main olfactory bulb	Present
TS25: medulla oblongata	Present
TS25: telencephalon ventricular layer	Present
TS25: corpus striatum	Weak
TS25: hippocampus marginal layer	Weak
TS25: pons	Weak
TS25: rhombic lip	Weak
TS25: superior colliculus	Weak
TS25: thalamus	Weak

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Strong
TS28: central lateral thalamic nucleus	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: lateral geniculate nucleus	Strong
TS28: main olfactory bulb	Strong
TS28: medial dorsal thalamic nucleus	Strong
TS28: pallidum	Strong
TS28: thalamus	Strong
TS28: olfactory tract	Present	Regionally restricted		Expression in latearal olfactory tract.
TS28: cerebellum	Present
TS28: cerebellum molecular layer	Present
TS28: cortical layer VI	Present
TS28: deep cerebellar nucleus	Present
TS28: frontal cortex	Present
TS28: inferior colliculus	Present
TS28: infralimbic area	Present
TS28: lateral dorsal thalamic nucleus	Present
TS28: mammillary body	Present
TS28: medulla oblongata	Present
TS28: midbrain	Present
TS28: neocortex	Present
TS28: olfactory tubercle	Present
TS28: parafascicular nucleus	Present
TS28: paraflocculus	Present
TS28: piriform cortex	Present
TS28: pons	Present
TS28: pontine nucleus	Present
TS28: primary motor cortex	Present
TS28: primary somatosensory cortex	Present
TS28: red nucleus	Present
TS28: ventral medial thalamic nucleus	Present
TS28: ventral tegmental area	Present
TS28: taenia tecta	Present	Regionally restricted		Expression in dorsal. Weak ventral expression.
TS28: accumbens nucleus	Weak
TS28: anterior medial cortex	Weak
TS28: auditory cortex	Weak
TS28: basolateral amygdaloid nucleus	Weak
TS28: bed nucleus of stria terminalis	Weak
TS28: caudate-putamen	Weak
TS28: central amygdaloid nucleus	Weak
TS28: cerebellum external granule cell layer	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: cingulate cortex	Weak
TS28: corpus striatum	Weak
TS28: cortical amygdaloid nucleus	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Weak
TS28: cuneate nucleus	Weak
TS28: dentate gyrus granule cell layer	Weak
TS28: dorsomedial hypothalamic nucleus	Weak
TS28: ectorhinal area	Weak
TS28: endopiriform nucleus	Weak
TS28: entorhinal cortex	Weak
TS28: globus pallidus	Weak
TS28: hippocampus CA1	Weak
TS28: hippocampus CA3	Weak
TS28: hippocampus pyramidal cell layer	Weak
TS28: lateral amygdaloid nucleus	Weak
TS28: lateral habenular nucleus	Weak
TS28: lateral posterior thalamic nucleus	Weak
TS28: lateral septal nucleus	Weak
TS28: medial amygdaloid nucleus	Weak
TS28: medial habenular nucleus	Weak
TS28: orbital area	Weak
TS28: perirhinal cortex	Weak
TS28: primary visual cortex	Weak
TS28: secondary motor cortex	Weak
TS28: solitary tract nucleus	Weak
TS28: somatic motor system	Weak
TS28: subiculum	Weak
TS28: substantia nigra	Weak
TS28: superior colliculus	Weak
TS28: temporal cortex association area	Weak
TS28: vestibular nucleus	Weak