Gene Expression Data
Assay Details
Assay
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Reference:
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J:239949
Amakawa Y, et al., A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects. Development. 2015 Dec 15;142(24):4299-308
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6368210
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Gene symbol:
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Xist
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Gene name:
|
inactive X specific transcripts
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Probe:
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pR97E1
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Assay notes:
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Probe was labeled with green-dUTP.
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Results
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Specimen
1C XX embryonic:
embryonic day 7.5
(more )
Specimen
1C XX embryonic:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
1C XCAG-X embryonic:
embryonic day 7.5; Xisttm6.1Sado/Xist +
(more )
Specimen
1C XCAG-X embryonic:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
1C XCAG-Y embryonic:
embryonic day 7.5; Xisttm6.1Sado/Y
(more )
Specimen
1C XCAG-Y embryonic:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 7.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
1C XX extraembryonic:
embryonic day 7.5
(more )
Specimen
1C XX extraembryonic:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
1C XCAG-X extraembryonic:
embryonic day 7.5; Xisttm6.1Sado/Xist +
(more )
Specimen
1C XCAG-X extraembryonic:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
1C XCAG-Y extraembryonic:
embryonic day 7.5; Xisttm6.1Sado/Y
(more )
Specimen
1C XCAG-Y extraembryonic:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 7.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2A XX 8-cell:
embryonic day 2.5
(more )
Specimen
2A XX 8-cell:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 2.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2A XCAG-X 8-cell:
embryonic day 2.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2A XCAG-X 8-cell:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
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Age:
|
embryonic day 2.5
|
Sex:
|
Female
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Type:
|
whole mount
|
Specimen
2A XY 8-cell:
embryonic day 2.5
(more )
Specimen
2A XY 8-cell:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 2.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2A XCAG-Y 8-cell:
embryonic day 2.5; Xisttm6.1Sado/Y
(more )
Specimen
2A XCAG-Y 8-cell:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
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Age:
|
embryonic day 2.5
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Sex:
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Male
|
Type:
|
whole mount
|
Specimen
2A XX blastocyst:
embryonic day 3.5
(more )
Specimen
2A XX blastocyst:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2A XCAG-X blastocyst:
embryonic day 3.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2A XCAG-X blastocyst:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2A XY blastocyst:
embryonic day 3.5
(more )
Specimen
2A XY blastocyst:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 3.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2A XCAG-Y blastocyst:
embryonic day 3.5; Xisttm6.1Sado/Y
(more )
Specimen
2A XCAG-Y blastocyst:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 3.5
|
Sex:
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Male
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Type:
|
whole mount
|
Specimen
2B E2.5 +/+:
embryonic day 2.5
(more )
Specimen
2B E2.5 +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 2.5
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Sex:
|
Female
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Type:
|
whole mount
|
Specimen
2B E2.5 XistCAG/+:
embryonic day 2.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E2.5 XistCAG/+:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 2.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E2.5 XistCAG/Y:
embryonic day 2.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E2.5 XistCAG/Y:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 2.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2B E3.5 +/+:
embryonic day 3.5
(more )
Specimen
2B E3.5 +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E3.5 XistCAG/+:
embryonic day 3.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E3.5 XistCAG/+:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E3.5 XistCAG/Y:
embryonic day 3.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E3.5 XistCAG/Y:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
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Age:
|
embryonic day 3.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2B E4.5 +/+:
embryonic day 4.5
(more )
Specimen
2B E4.5 +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 4.5
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Sex:
|
Female
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Type:
|
whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS6: embryo |
Present |
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2B E4.5 +/+
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Most embryos exhibit monoallelic expression.
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Specimen
2B E4.5 XistCAG/+:
embryonic day 4.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E4.5 XistCAG/+:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 4.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E4.5 XistCAG/Y:
embryonic day 4.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E4.5 XistCAG/Y:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 4.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS6: embryo |
Present |
|
2B E4.5 XistCAG/Y
|
Most embryos exhibit no expression and <20% exhibit monoallelic expression.
|
Specimen
2B E6.5 +/+ emb:
embryonic day 6.5
(more )
Specimen
2B E6.5 +/+ emb:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 6.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E6.5 +/+ troph:
embryonic day 6.5
(more )
Specimen
2B E6.5 +/+ troph:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 6.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E6.5 XistCAG/+ emb:
embryonic day 6.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E6.5 XistCAG/+ emb:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 6.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E6.5 XistCAG/+ troph:
embryonic day 6.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E6.5 XistCAG/+ troph:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 6.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E6.5 XistCAG/Y emb:
embryonic day 6.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E6.5 XistCAG/Y emb:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 6.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2B E6.5 XistCAG/Y troph:
embryonic day 6.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E6.5 XistCAG/Y troph:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 6.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2B E7.5 +/+ emb:
embryonic day 7.5
(more )
Specimen
2B E7.5 +/+ emb:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E7.5 +/+ troph:
embryonic day 7.5
(more )
Specimen
2B E7.5 +/+ troph:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E7.5 XistCAG/+ emb:
embryonic day 7.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E7.5 XistCAG/+ emb:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E7.5 XistCAG/+ troph:
embryonic day 7.5; Xisttm6.1Sado/Xist +
(more )
Specimen
2B E7.5 XistCAG/+ troph:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 7.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
2B E7.5 XistCAG/Y emb:
embryonic day 7.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E7.5 XistCAG/Y emb:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 7.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
2B E7.5 XistCAG/Y troph:
embryonic day 7.5; Xisttm6.1Sado/Y
(more )
Specimen
2B E7.5 XistCAG/Y troph:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 7.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Specimen
3A:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 2.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
3B E3.5:
embryonic day 3.5
(more )
Note: Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
Specimen
3B E3.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
|
Specimen
3B E4.5:
embryonic day 4.5
(more )
Note: Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
Specimen
3B E4.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 4.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
|
Specimen
3B E3.5 XXCAG:
embryonic day 3.5; Xisttm6.1Sado/Xist +
(more )
Note: Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
Specimen
3B E3.5 XXCAG:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 3.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
|
Specimen
3B E4.5 XXCAG:
embryonic day 4.5; Xisttm6.1Sado/Xist +
(more )
Note: Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
Specimen
3B E4.5 XXCAG:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 4.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
|
Specimen
S3 XCAG-X:
embryonic day 2.5; Xisttm6.1Sado/Xist +
(more )
Specimen
S3 XCAG-X:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Xist+
|
Age:
|
embryonic day 2.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Specimen
S3 XCAG-Y:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Xisttm6.1Sado/Y
|
Age:
|
embryonic day 2.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
|