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Gene Expression Data
Assay Details
Assay
Reference: J:239949 Amakawa Y, et al., A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects. Development. 2015 Dec 15;142(24):4299-308
Assay type: RNA in situ
MGI Accession ID: MGI:6368210
Gene symbol: Xist
Gene name: inactive X specific transcripts
Probe: pR97E1
Probe preparation: Antisense labelled with fluorescein RNA
Assay notes: Probe was labeled with green-dUTP.
Results
Specimen 1C XX embryonic: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 1C XX embryonic
Specimen 1C XCAG-X embryonic: embryonic day 7.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 1C XCAG-X embryonic There is biallelic expression in this mutant.
Specimen 1C XCAG-Y embryonic: embryonic day 7.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 1C XCAG-Y embryonic
Specimen 1C XX extraembryonic: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 1C XX extraembryonic
Specimen 1C XCAG-X extraembryonic: embryonic day 7.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 1C XCAG-X extraembryonic There is ectopic expression in this mutant.
Specimen 1C XCAG-Y extraembryonic: embryonic day 7.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 1C XCAG-Y extraembryonic
Specimen 2A XX 8-cell: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2A XX 8-cell
Specimen 2A XCAG-X 8-cell: embryonic day 2.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2A XCAG-X 8-cell
Specimen 2A XY 8-cell: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2A XY 8-cell
Specimen 2A XCAG-Y 8-cell: embryonic day 2.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2A XCAG-Y 8-cell
Specimen 2A XX blastocyst: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A XX blastocyst
Specimen 2A XCAG-X blastocyst: embryonic day 3.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A XCAG-X blastocyst There is biallelic ectopic expression in this mutant.
Specimen 2A XY blastocyst: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Absent 2A XY blastocyst
Specimen 2A XCAG-Y blastocyst: embryonic day 3.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A XCAG-Y blastocyst There is ectopic expression in this mutant.
Specimen 2B E2.5 +/+: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B E2.5 +/+ Most embryos exhibit monoallelic expression.
Specimen 2B E2.5 XistCAG/+: embryonic day 2.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B E2.5 XistCAG/+ All embryos exhibit monoallelic expression.
Specimen 2B E2.5 XistCAG/Y: embryonic day 2.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2B E2.5 XistCAG/Y
Specimen 2B E3.5 +/+: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B E3.5 +/+ Most embryos exhibit monoallelic expression.
Specimen 2B E3.5 XistCAG/+: embryonic day 3.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B E3.5 XistCAG/+ Most embryos exhibit monoallelic expression.
Specimen 2B E3.5 XistCAG/Y: embryonic day 3.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B E3.5 XistCAG/Y Most embryos exhibit no expression and <20% exhibit monoallelic expression.
Specimen 2B E4.5 +/+: embryonic day 4.5 (more )
Structure Level Pattern Image Note
TS6: embryo Present 2B E4.5 +/+ Most embryos exhibit monoallelic expression.
Specimen 2B E4.5 XistCAG/+: embryonic day 4.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS6: embryo Present 2B E4.5 XistCAG/+ Most embryos exhibit monoallelic expression.
Specimen 2B E4.5 XistCAG/Y: embryonic day 4.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS6: embryo Present 2B E4.5 XistCAG/Y Most embryos exhibit no expression and <20% exhibit monoallelic expression.
Specimen 2B E6.5 +/+ emb: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 2B E6.5 +/+ emb Most embryos exhibit monoallelic expression.
Specimen 2B E6.5 +/+ troph: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: trophectoderm Present 2B E6.5 +/+ troph Most embryos exhibit monoallelic expression.
Specimen 2B E6.5 XistCAG/+ emb: embryonic day 6.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 2B E6.5 XistCAG/+ emb Most embryos exhibit monoallelic expression.
Specimen 2B E6.5 XistCAG/+ troph: embryonic day 6.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS9: trophectoderm Present 2B E6.5 XistCAG/+ troph Most embryos exhibit biallelic expression.
Specimen 2B E6.5 XistCAG/Y emb: embryonic day 6.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 2B E6.5 XistCAG/Y emb Most embryos exhibit monoallelic expression and about 30% no expression.
Specimen 2B E6.5 XistCAG/Y troph: embryonic day 6.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS9: trophectoderm Present 2B E6.5 XistCAG/Y troph Most embryos exhibit monoallelic expression and about 25% no expression.
Specimen 2B E7.5 +/+ emb: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 2B E7.5 +/+ emb Most embryos exhibit monoallelic expression.
Specimen 2B E7.5 +/+ troph: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 2B E7.5 +/+ troph Most embryos exhibit monoallelic expression.
Specimen 2B E7.5 XistCAG/+ emb: embryonic day 7.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 2B E7.5 XistCAG/+ emb Most embryos exhibit monoallelic expression.
Specimen 2B E7.5 XistCAG/+ troph: embryonic day 7.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 2B E7.5 XistCAG/+ troph Most embryos exhibit biallelic expression.
Specimen 2B E7.5 XistCAG/Y emb: embryonic day 7.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 2B E7.5 XistCAG/Y emb Most embryos exhibit monoallelic expression.
Specimen 2B E7.5 XistCAG/Y troph: embryonic day 7.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS11: trophectoderm Present 2B E7.5 XistCAG/Y troph Most embryos exhibit monoallelic expression.
Specimen 3A: embryonic day 2.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3A
Specimen 3B E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B E3.5 XX , 3B E3.5 XX Merge
Specimen 3B E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
Structure Level Pattern Image Note
TS6: embryo Present 3B E4.5 XX , 3B E4.5 XX Merge
Specimen 3B E3.5 XXCAG: embryonic day 3.5; Xisttm6.1Sado/Xist+ (more )
Note: Double labeled: red - Xist; green - Nanog (assay MGI:6368215).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B E3.5 XXCAG , 3B E3.5 XXCAG Merge
Specimen 3B E4.5 XXCAG: embryonic day 4.5; Xisttm6.1Sado/Xist+ (more )
Note: Double labeled: red - Xist; green - Pou5f1 (assay MGI:6368216).
Structure Level Pattern Image Note
TS6: embryo Present 3B E4.5 XXCAG Merge , 3B E4.5 XXCAG
Specimen S3 XCAG-X: embryonic day 2.5; Xisttm6.1Sado/Xist+ (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present S3_XCAG-X merge , S3_XCAG-X
Specimen S3 XCAG-Y: embryonic day 2.5; Xisttm6.1Sado/Y (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present S3_XCAG-Y merge , S3_XCAG-Y
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory