Xist^tm6.1Sado
Targeted Allele Detail

Summary

• Symbol: Xist^tm6.1Sado
• Name: inactive X specific transcripts; targeted mutation 6.1, Takashi Sado
• MGI ID: MGI:5882209
• Synonyms: Xist^CAG
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Xist^tm6.1Sado page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239949
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutation: Insertion
• Mutation details: A CAG promoter and a loxP site flanked puromycin resistance gene and ECFP reporter gene double cassette were inserted upstream of exon 1 replacing the native promoter. Subsequent cre-mediated recombination removed the selection/reporter cassette, bringing the gene under the constitutively active control of the CAG promoter. The expression from this allele is around tenfold higher than from the wild-type allele. The half-life of the transcripts is the same as wild-type. (J:239949)

Expression

In Mice Carrying this Mutation:

29 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 8 strains or lines available

References

• Original: J:239949 Amakawa Y, et al., A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects. Development. 2015 Dec 15;142(24):4299-308
• All: 2 reference(s)