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Gene Expression Data
Assay Details
Assay
Reference: J:241573 Pui HP, et al., Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mech Dev. 2017 Apr;144(Pt B):125-139
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6305364
Gene symbol: Zbtb16
Gene name: zinc finger and BTB domain containing 16
Antibody: Anti-PLZF
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1B E17.5: embryonic day 17.5 (more )
Note: Double labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359).
Structure Level Pattern Image Note
TS25: germ cell of testis Absent 1B E17.5 PLZF+ECAD
Specimen 1B E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359).
Structure Level Pattern Image Note
TS26: seminiferous cord Present 1B E18.5 PLZF+ECAD Expression was detected in nascent spermatogonia.
Specimen 1B P0.5: postnatal day 0.5 (more )
Note: Double labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359).
Structure Level Pattern Image Note
TS27: spermatogonium Present 1B P0.5 PLZF+ECAD
Specimen 1B P1.5: postnatal day 1.5 (more )
Note: Double labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359).
Structure Level Pattern Image Note
TS27: spermatogonium Present 1B P1.5 PLZF+ECAD
Specimen 2A top: embryonic day 18.5 (more )
Note: Double labeled: green - Zbtb16; red - Gfra1 (assay MGI:6305361).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 2A GFRAI+PLZF , 2A PLZF top Expression was detected in nascent spermatogonia, which co-expressed Gfra1.
Specimen 2A middle: embryonic day 18.5 (more )
Note: Double labeled: green - Zbtb16; red - Ret (assay MGI:6306308).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 2A PLZF middle , 2A RET+PLZF Expression was detected in nascent spermatogonia, which co-expressed Ret.
Specimen 2A bottom: embryonic day 18.5 (more )
Note: Double labeled: green - Zbtb16; red - Stra8 (assay MGI:6305367).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 2A Stra8+PLZF , 2A PLZF bottom Expression was detected in nascent spermatogonia, a subset of which co-expressed Stra8.
Specimen 2B E17.5: embryonic day 17.5 (more )
Note: Triple labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS25: germ cell of testis Absent 2B E17.5 PLZF , 2B E17.5 PLZF+ECAD+Laminin
Specimen 2B E18.5: embryonic day 18.5 (more )
Note: Triple labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS26: germ cell of testis Present Regionally restricted 2B E18.5 PLZF , 2B E18.5 PLZF+ECAD+Laminin At E18.5, the majority of the re-located gonocytes did not express Zbtb16.
Specimen 2B P0.5: postnatal day 0.5 (more )
Note: Triple labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 2B P0.5 PLZF , 2B P0.5 PLZF+ECAD+Laminin The proportion of Zbtb16-immunopositive relocated gonocytes increased after birth.
Specimen 2B P1.5: postnatal day 1.5 (more )
Note: Triple labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 2B P1.5 PLZF+ECAD+Laminin , 2B P1.5 PLZF The majority of relocated gonocytes were immunopositive for Zbtb16 at P1.5.
Specimen S1C: postnatal day 1.5 (more )
Note: Triple labeled: green - Zbtb16; red - Cdh1 (assay MGI:6305359); white - PH3.
Structure Level Pattern Image Note
TS27: germ cell of testis Present S1C PLZF , S1C P1.5 top The majority of mitotic germ cells expressed Zbtb16.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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