Gene Expression Data
Assay Details
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Assay
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Reference:
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J:140105
Li L, et al., A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell. 2008 Sep;15(3):416-25
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6286514
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Gene symbol:
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Tle6
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Gene name:
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transducin-like enhancer of split 6
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Antibody:
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anti-Tle6
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Detection system:
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Secondary antibody
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Results
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Specimen
5A Egg:
postnatal
(more  )
Note: Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Egg:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Female
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Type:
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whole mount
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Note:
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Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Present |
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5A Egg Tle6
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5A Egg Merge
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Expressed in the subcortex, colocalized with Ooep and Nlrp5.
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Specimen
5A 1-Cell:
embryonic day 0.5
(more )
Note: Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
Specimen
5A 1-Cell:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage conceptus |
Present |
Regionally restricted |
5A 1-cell Merge
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5A 1-cell Tle6
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Colocalized in the subcortex with Ooep and Nlrp5. Expression was attenuated where the zygotic membrane was in contact with polar bodies.
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Specimen
5A 2-Cell:
embryonic day 1.0
(more )
Note: Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
Specimen
5A 2-Cell:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage conceptus |
Present |
Regionally restricted |
5A 2-cell Merge
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5A 2-cell Tle6
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Expression was excluded from regions of cell-cell contact. Colocalized in the subcortex with Ooep and Nlrp5.
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Specimen
5A Morula:
embryonic day 2.5
(more )
Note: Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Morula:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
5A Morula Merge
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5A Morula Tle6
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Expression was excluded from the inner cells of the morula. Colocalized in the subcortex with Nlrp5 and Ooep.
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Specimen
5A Blastocyst:
embryonic day 3.5
(more )
Note: Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Blastocyst:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: purple - Tle6; green - Ooep (assay MGI:6286406); red - Nlrp5 (assay MGI:6286513).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Present |
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5A Blastocyst Merge
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5A Blastocyst Tle6
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Colocalized in the subcortex with Nlrp5 and Ooep.
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| TS4: inner cell mass |
Absent |
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5A Blastocyst Merge
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5A Blastocyst Tle6
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Analysis Tools