Antibody:	anti-Mater
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		5A Egg Merge , 5A Egg Mater	Expressed in the subcortex, colocalized with Ooep and Tle6.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage conceptus	Present	Regionally restricted	5A 1-cell Mater , 5A 1-cell Merge	Colocalized in the subcortex with Ooep and Tle6. Expression was attenuated where the zygotic membrane was in contact with polar bodies.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage conceptus	Present	Regionally restricted	5A 2-cell Merge , 5A 2-cell Mater	Expression was excluded from regions of cell-cell contact. Colocalized in the subcortex with Ooep and Tle6.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present	Regionally restricted	5A Morula Mater , 5A Morula Merge	Expression was excluded from the inner cells of the morula. Colocalized in the subcortex with Tle6 and Ooep.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		5A Blastocyst Merge , 5A Blastocyst Mater	Colocalized in the subcortex with Tle6 and Ooep.
TS4: inner cell mass	Absent		5A Blastocyst Merge , 5A Blastocyst Mater

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		6B left	Expressed in the subcortex.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Weak	Diffuse	6B middle	Expressed in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Absent		6B right