Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:140105
Li L, et al., A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell. 2008 Sep;15(3):416-25
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6286406
|
|
Gene symbol:
|
Ooep
|
|
Gene name:
|
oocyte expressed protein
|
|
Antibody:
|
anti-Floped
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
S2C Null:
(close  )
Specimen
S2C Control:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
OoepGt(139A2)Cmhd/Ooep+
|
|
Age:
|
postnatal
|
|
Sex:
|
Female
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
|
S2C right
|
|
Specimen
5A Egg:
postnatal
(more  )
Note: Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Egg:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Present |
|
5A Egg Floped
,
5A Egg Merge
|
Expressed in the subcortex and extended further into the cytoplasm. Colocalized in the subcortex with Nlrp5 and Tle6.
|
Specimen
5A 1-Cell:
embryonic day 0.5
(more )
Note: Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
Specimen
5A 1-Cell:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 0.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage conceptus |
Present |
Regionally restricted |
5A 1-cell Merge
,
5A 1-cell Floped
|
Colocalized in the subcortex with Nlrp5 and Tle6. Expression was attenuated where the zygotic membrane was in contact with polar bodies.
|
Specimen
5A 2-Cell:
embryonic day 1.0
(more )
Note: Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
Specimen
5A 2-Cell:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage conceptus |
Present |
Regionally restricted |
5A 2-cell Merge
,
5A 2-cell Floped
|
Expression was excluded from regions of cell-cell contact. Colocalized in the subcortex with Nlrp5 and Tle6.
|
Specimen
5A Morula:
embryonic day 2.5
(more )
Note: Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Morula:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
5A Morula Floped
,
5A Morula Merge
|
Expression was excluded from the inner cells of the morula. Colocalized in the subcortex with Nlrp5 and Tle6.
|
Specimen
5A Blastocyst:
embryonic day 3.5
(more )
Note: Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
Specimen
5A Blastocyst:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Ooep; purple - Tle6 (assay MGI:6286514); red - Nlrp5 (assay MGI:6286513).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Present |
|
5A Blastocyst Merge
,
5A Blastocyst Floped
|
Colocalized in the subcortex with Nlrp5 and Tle6.
|
| TS4: inner cell mass |
Absent |
|
5A Blastocyst Merge
,
5A Blastocyst Floped
|
|
Specimen
5C Control:
embryonic day 3.5; Tead4tm1.1Bnno/Tead4 +
(more )
Note: Double labeled: green - Ooep; red - Pou5f1 (assay MGI:6286516).
Specimen
5C Control:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Tead4tm1.1Bnno/Tead4+
|
|
Age:
|
embryonic day 3.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Double labeled: green - Ooep; red - Pou5f1 (assay MGI:6286516).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: trophectoderm |
Present |
|
5C upper left
,
5C upper middle
,
5C upper merge
|
|
Specimen
5C Tead4 tm/tm:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Tead4tm1.1Bnno/Tead4tm1.1Bnno
|
|
Age:
|
embryonic day 3.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Double labeled: green - Ooep; red - Pou5f1 (assay MGI:6286516).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: conceptus |
Present |
Regionally restricted |
5C lower middle
,
5C lower merge
,
5C lower left
|
Null embryos did not form blastocysts but remained as a clump of cells. Expression was restricted to outer cells.
|
|
Analysis Tools