Gene Expression Data
Assay Details
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Assay
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Reference:
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J:263439
Deans MR, et al., Mammalian Otolin: a multimeric glycoprotein specific to the inner ear that interacts with otoconial matrix protein Otoconin-90 and Cerebellin-1. PLoS One. 2010 Sep 15;5(9):e12765
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6275832
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Gene symbol:
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Otol1
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Gene name:
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otolin 1
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Antibody:
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Anti-Otolin
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
5D:
postnatal day 2
(more  )
Note: Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
Specimen
5D:
(close  )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: saccule |
Present |
Regionally restricted |
5D
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Immunofluorescent labeling showed little colocalization of Otol1 with the hair cell marker Calb2, indicating that expression of Otol1 is in the support cells.
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Specimen
5F:
postnatal day 2
(more )
Note: Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
Specimen
5F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear epithelium |
Present |
Regionally restricted |
5F
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5F'
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Immunofluorescent Otol1 labeling in the support cell processes located between the Calb2-immunopositive hair cells.
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Specimen
5G:
postnatal day 2
(more )
Note: Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
Specimen
5G:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Otol1; green - Calb2 (assay MGI:6275835).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: crista ampullaris |
Present |
Regionally restricted |
5G
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Immunofluorescent Otol1 labeling in the support cell processes located between the Calb2-immunopositive hair cells.
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Specimen
6F:
embryonic day 18.5
(more )
Specimen
6F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
Regionally restricted |
6F
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Expression was visualized in inner phalangeal cells and border cells. Hair cells were not labeled.
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| TS26: stria vascularis |
Present |
Regionally restricted |
6F
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Expression was detected in the marginal cells of the stria vascularis.
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| TS26: external spiral sulcus |
Present |
Regionally restricted |
6F
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Expression was detected in a small population of cells in the outer sulcus.
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Specimen
6G:
embryonic day 18.5
(more )
Note: Double labeled: red - Otol1; green - phalloidin.
Specimen
6G:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Otol1; green - phalloidin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
Regionally restricted |
6G
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6G'
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Expression was detected at the tips of the sterocilia bundle.
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Analysis Tools