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Gene Expression Data
Assay Details
Assay
Reference: J:167064 L'honore A, et al., Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development. 2010 Nov;137(22):3847-56
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6260202
Gene symbol: Myf5
Gene name: myogenic factor 5
Reporter: lacZ
Antibody: anti-B-galactosidase (Sigma)
Detection system: Alexa Fluor 488
Results
Specimen 3B/C: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: limb bud Present 3B , 3C
Specimen 3E/F: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: limb bud Present 3E , 3F Authors report almost no co-labeling with MyoD, compared with control littermates.
Specimen S2H/I: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: green - Myf5 reporter; red - Pax3 (assay MGI:6260190).
Structure Level Pattern Image Note
TS19: limb bud Present S2H , S2I
Specimen S2K/L: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: green - Myf5 reporter; red - Pax3 (assay MGI:6260190).
Structure Level Pattern Image Note
TS19: limb bud Present S2K , S2L Authors state the number of Myf5/Pax3 double positive cells was similar in Pitx2-deficient limb buds.
Specimen 5I: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5I
Specimen 5J: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5J Double Pitx2 and Myf5(Mrf) loss-of-function did not appear to affect the number of expressing cells.
Specimen 5K: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5K
Specimen 5L: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: green - Myf5 reporter; red - Myod1 (assay MGI:6260194).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5L Double Pitx2 and Myf5(Mrf) loss-of-function did not appear to affect the number of expressing cells.
Specimen 6F: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 6F Expression in myogenic precursors.
Specimen 6H: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 6H Authors state loss of Pitx2 did not affect the appearance of myotome cells.
Specimen S6G: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas (more )
Note: Double labeled: green - Myf5 reporter; red - Myog (assay MGI:6260198).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted S6G Expression in myogenic precursors.
Specimen S6H: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: green - Myf5 reporter; red - Myog (assay MGI:6260198).
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted S6H Authors state loss of Pitx2 did not affect the appearance of myotome cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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