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Gene Expression Data
Assay Details
Assay
Reference: J:167064 L'honore A, et al., Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development. 2010 Nov;137(22):3847-56
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6260190
Gene symbol: Pax3
Gene name: paired box 3
Antibody: anti-Pax3 (clone C2)
Detection system: Alexa Fluor 555
Results
Specimen 1B/C: embryonic day 11.0; Myf5tm2Pas/Myf5+ (more )
Note: Double labeled: red - Pax3; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS18: forelimb bud Present 1C , 1B

Specimen S2A/C: embryonic day 11.5; Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: red - Pax3; green - Ki67 (Mki67 assay MGI:6260199).
Structure Level Pattern Image Note
TS19: limb bud Present S2C , S2A

Specimen S2D/F: embryonic day 11.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Pax3; green - Ki67 (Mki67 assay MGI:6260199).
Structure Level Pattern Image Note
TS19: limb bud Present S2D , S2F Authors report the number of expressing cells is unaltered in mutant limb buds.

Specimen S2G/I: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: red - Pax3; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: limb bud Present S2I , S2G

Specimen S2J/L: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Pax3; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: limb bud Present S2J , S2L Authors state the number of Myf5/Pax3 double positive cells was similar in Pitx2-deficient limb buds.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory