Dnmt1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:135338 Cirio MC, et al., Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol. 2008;8:9
Assay type:	Western blot
MGI Accession ID:	MGI:6201237
Gene symbol:	Dnmt1
Gene name:	DNA methyltransferase 1

Antibody:	UPT82
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Antibody recognizes only the Dnmt1s proteins.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
+ GV	P21-35	TS28: primary oocyte	Present (a)	Not Specified		Female
+ MII	P21-35	TS28: secondary oocyte	Present	Not Specified		Female
1s/1o MII	P21-35	TS28: secondary oocyte	Present (b)	involves: 129S1/Sv * C57BL/6	Dnmt1^tm1Jamt/Dnmt1^tm1Jamt	Female
delta1o MII	P21-35	TS28: secondary oocyte	Present (c)	involves: 129S1/Sv * FVB/N	Dnmt1^tm1Jrc/Dnmt1^tm1Jrc	Female

Notes:
(a) Present at an approximately 10-fold higher concentration in GV-stage oocytes compared to MII oocytes.
(b) Increased expression compared to the wild-type.
(c) Increased expression (40-fold) compared to the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24