Probe:	rspo3 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2A	Expression in dorsal telencephalon.
TS15: tail	Present		2A

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2B	Expression in dorsal telencephalon.
TS15: atrioventricular canal	Present		2B
TS15: tail	Present		2B

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2C	Expression in medial wall of dorsal telencephalon, in a domain surrounding the boundary between telencephalon and diencephalon.
TS17: central nervous system roof plate	Present	Widespread	2C	Expression also detected in roof plate throughout entire neural tube caudal to telencephalic vesicles.
TS17: otocyst	Present		2C
TS17: somite	Present		2C
TS17: tail	Present		2C

Structure	Level	Pattern	Image	Note
TS17: central nervous system roof plate	Present	Widespread	2D	Expression also detected in roof plate throughout entire neural tube caudal to telencephalic vesicles.
TS17: head mesenchyme	Present		2D

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2E	Expression in medial wall of dorsal telencephalon, in a domain surrounding the boundary between telencephalon and diencephalon.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present		2F
TS17: truncus arteriosus	Present		2F

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		2G
TS17: medial-nasal process	Present		2G

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		2H
TS17: otocyst	Present		2H
TS17: forelimb bud	Present	Regionally restricted	2H	Expression in forelimb and hindlimbs - stripe of expression stretching along proximal-distal axis in caudal domain, but not at caudal edge.
TS17: hindlimb bud	Present	Regionally restricted	2H	Expression in forelimb and hindlimbs - stripe of expression stretching along proximal-distal axis in caudal domain, but not at caudal edge.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2I	Arch-shaped expression pattern parallel to apical ectodermal ridge.
TS19: hindlimb bud	Present	Regionally restricted	2I	Arch-shaped expression pattern parallel to apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Present		2J
TS20: hindlimb interdigital region	Present		2J

Structure	Level	Pattern	Image	Note
TS12: chorion	Weak		3I	Authors report expression coincided with Rspo3 reporter gene expression (assay MGI:6158433).
TS12: allantois	Trace		3I	Authors report expression coincided with Rspo3 reporter gene expression (assay MGI:6158433).

Structure	Level	Pattern	Image	Note
TS13: chorioallantoic placenta	Present	Regionally restricted	3J	Authors report expression coincided with Rspo3 reporter gene expression (assay MGI:6158433).

Structure	Level	Pattern	Image	Note
TS17: chorioallantoic placenta	Present	Regionally restricted	3L	Authors report expression coincided with Rspo3 reporter gene expression (assay MGI:6158433).