Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119866
Bort R, et al., Hex homeobox gene controls the transition of the endoderm to a pseudostratified, cell emergent epithelium for liver bud development. Dev Biol. 2006 Feb 1;290(1):44-56
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6157941
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA3
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Assay notes:
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It is possible that another Shh probe (MGI:1929246) was used in this assay.
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Results
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Specimen
7A:
embryonic day 8.5
(more )
Note: 12 somites (stage II)
Specimen
7A:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites (stage II)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: gut endoderm |
Present |
Regionally restricted |
7A
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No expression in wild-type liver bud.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Hhextm1Rbe/Hhextm1Rbe
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites (stage II)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: gut endoderm |
Present |
Regionally restricted |
7B
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Expression expanded ventro-laterally in liver region.
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Specimen
7C:
embryonic day 8.5
(more )
Note: 12 somites (stage II)
Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites (stage II)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: gut endoderm |
Present |
Regionally restricted |
7C
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No expression at level of anterior intestinal portal, caudal to liver region.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Hhextm1Rbe/Hhextm1Rbe
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites (stage II)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: gut endoderm |
Present |
Regionally restricted |
7D
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Ectopic expression in ventral endoderm spanning the sinus venosus region.
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Specimen
7E:
embryonic day 9.0
(more )
Note: 16 somites (late stage II)
Specimen
7E:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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16 somites (late stage II)
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Hhextm1Rbe/Hhextm1Rbe
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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16 somites (late stage II)
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