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Gene Expression Data
Assay Details
Assay
Reference: J:28395 Kaneko-Ishino T, et al., Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization. Nat Genet. 1995 Sep;11(1):52-9
Assay type: RT-PCR
MGI Accession ID: MGI:6154604
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest-pA, Mest-pB
Assay notes: Imprinting verification by RFLP analysis. RT-PCR products were digested with TaqI prior to electrophoresis.
Results Image: 5b
 Sample Information Bands Other Sample Information
Lane AgeStructure 284 bp (a) 284 bp (b) 203 bp (a) 173 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
M Mol. Wt. Marker Lane
1 P adult TS28: liver Present Not Applicable Present Not Applicable 0.1 µg; total RNA M. spretus Male
2 P adult TS28: liver Not Applicable Present Not Applicable Present 0.1 µg; total RNA C57BL/6 Female
3 E9.5 TS15: embryo Present (c) Absent (d) Present (c) Absent (d) 0.1 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
4 E9.5 TS15: embryo Present (c) Absent (d) Present (c) Absent (d) 0.1 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
5 E9.5 TS15: embryo Present (c) Absent (d) Present (c) Absent (d) 0.1 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
M Mol. Wt. Marker Lane
Notes:
(a) Mus spretus band.
(b) C57BL/6 band.
(c) Paternal band was present.
(d) Maternal band was absent.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory