Probe:	Evx2 probe2
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography
Assay notes:	Experiments were performed both on paraffin-embedded embryo section and frozen section. It was not specified which was used.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		2A middle

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		2B middle

Structure	Level	Pattern	Image	Note
TS17: tail	Absent		2C middle

Structure	Level	Pattern	Image	Note
TS15: hindgut epithelium	Present	Regionally restricted	3A middle	Expressed in the cloacal epithelium.
TS15: hindgut mesenchyme	Present	Regionally restricted	3A middle	Expressed in the mesenchyme surrounding the cloacal epithelium.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Absent		3B middle

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3C middle	Expressed in a subset of Hoxd13-expressing cells in distal and posterior mesenchyme, within an area that also expressed Evx1.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted	3D middle	Expressed in a Hoxd-type of expression pattern, that is, across a wider distal region.
TS20: genital tubercle	Present		3D middle

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	4A left	Expressed in slightly less extended domains than those of Hoxd13, less proximally along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	4B left	Expressed in slightly less extended domains than those of Hoxd13, both less proximally and less anteriorly, along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	4C left	Expressed in slightly less extended domains than those of Hoxd13, both less proximally and less anteriorly, along the posterior margin of the bud.

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted	4D left	Expression was not as proximal as the Hoxd13 domain along the posterior margin and the anterior margin.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 1	Weak		4E left
TS21: hindlimb digit 2	Weak		4E left
TS21: hindlimb digit 3	Weak		4E left
TS21: hindlimb digit 4	Weak		4E left
TS21: hindlimb digit 5	Weak		4E left

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present		5A left
TS19: mesenchyme of urogenital sinus	Present		5A left
TS19: hindgut mesenchyme	Present	Regionally restricted	5A left	Expressed around the urogenital sinus and rectum.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present	Regionally restricted	5B left	Not detected in morelaterul regions of the genital bud.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present		5C left

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6A	Expressed in a thin and continuous column of cells along the entire spinal cord in near mid-sagittal sections.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6B	Expressed in more scattered cells of the ventral spinal cord in more lateral sections.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	6C middle	Expressed in two symmetrical columns in the medial part. Expression was weaker than that of Evx1.
TS20: spinal cord lateral wall	Present	Regionally restricted	6C middle	Expressed in cells located between the ventral horns and the ventricular zone and in the most dorsal layers.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	6D middle	Expressed in three components: a thin column of cells, group of ventral cells and the most superficial layer.
TS20: midbrain-hindbrain junction	Present		6D middle
TS20: midbrain	Present	Regionally restricted	6D middle	Expressed in the superficial layer of the entire midbrain.

Structure	Level	Pattern	Image	Note
TS19: hindbrain marginal layer	Present		7A right
TS19: midbrain marginal layer	Present		7A right

Structure	Level	Pattern	Image	Note
TS19: hindbrain marginal layer	Present		7B right
TS19: midbrain marginal layer	Present		7B right

Structure	Level	Pattern	Image	Note
TS15: limb bud	Absent

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud	Absent

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present			Expressed in discrete cell layers.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present			Expressed in a different pattern as Evx1.
TS19: limb bud	Present			Expressed in a different pattern as Evx1.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted		Expressed in two symmetrical columns in the medial part in more caudal regions of the spinal cord (thoracic and lumbar).
TS20: spinal cord lateral wall	Present	Regionally restricted		Expressed in cells located between the ventral horns in more caudal regions of the spinal cord (thoracic and lumbar).

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Trace			Hardly detectable.