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Gene Expression Data
Assay Details
Assay
Reference: J:161418 Takahashi J, et al., Analysis of Ripply1/2-deficient mouse embryos reveals a mechanism underlying the rostro-caudal patterning within a somite. Dev Biol. 2010 Jun 15;342(2):134-45
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5805858
Gene symbol: Tbx6
Gene name: T-box 6
Antibody: anti-Tbx6 (N-terminal)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: The authors did not specify which antibody was used, this may have been used instead: anti-Tbx6 (internal) MGI:7495530 . Horseradish peroxidase-conjugated donkey anti-rabbit IgG antibody was the secondary antibody and fluorescein isothiocyanate (FITC)-conjugated tyramide was used for detection.
Results
Specimen 5B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present 5B
Specimen 5D: embryonic day 11.5; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present 5D Protein has expanded anteriorly in this mutant.
Specimen 5F: embryonic day 11.5; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present 5F Protein has expanded anteriorly in this mutant.
Specimen 5G: embryonic day 11.5; Mesp2tm6Ysa/Mesp2tm6Ysa (more )
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present 5G Protein has expanded anteriorly in this mutant.
Specimen 5J,K: embryonic day 11.0 (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5K , 5J
Specimen 5N,O: embryonic day 11.0; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5O , 5N Protein has expanded anteriorly in this mutant.
Specimen 5R,S: embryonic day 11.0; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5R , 5S Protein has expanded anteriorly in this mutant.
Specimen S5A: embryonic day 11.0 (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5A NICD + Tbx6 , S5A Tbx6
Specimen S5B: embryonic day 11.0 (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5B Tbx6 , S5B NICD + Tbx6
Specimen S5C: embryonic day 11.0 (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5C NICD + Tbx6 , S5C Tbx6
Specimen S5D: embryonic day 11.0 (more )
Note: Double labeled: green - Tbx6; magenta - Notch1 (assay MGI:5805857).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5D NICD + Tbx6 , S5D Tbx6
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory