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Gene Expression Data
Assay Details
Assay
Reference: J:161418 Takahashi J, et al., Analysis of Ripply1/2-deficient mouse embryos reveals a mechanism underlying the rostro-caudal patterning within a somite. Dev Biol. 2010 Jun 15;342(2):134-45
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5805857
Gene symbol: Notch1
Gene name: notch 1
Antibody: anti-cleaved Notch1 (Val1744) antibody (anti-NICD) (#2421)
Detection system: Secondary antibody coupled to Cy3
Assay notes: The antibody recognizes the activated form of the protein. Horseradish peroxidase-conjugated donkey anti-rabbit IgG antibody was the secondary antibody and Cyanin3 tyramide was used for detection.
Results
Specimen 3A: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). A' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3A NICD + Mesp2 , 3A' , 3A NICD
Specimen 3B: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). B' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3B NICD + Mesp2 , 3B' , 3B NICD
Specimen 3C: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). C' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3C NICD , 3C' , 3C NICD + Mesp2
Specimen 3D: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). D' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3D' , 3D NICD , 3D NICD + Mesp2
Specimen 3E: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). E' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3E NICD , 3E' , 3E NICD + Mesp2
Specimen 3F: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). F' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3F' , 3F NICD + Mesp2 , 3F NICD
Specimen 3G: embryonic day 11.0; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). G' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3G NICD , 3G' , 3G NICD + Mesp2
Specimen 3H: embryonic day 11.0; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). H' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3H NICD + Mesp2 , 3H' , 3H NICD
Specimen 3I: embryonic day 11.0; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). I' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3I NICD , 3I' , 3I NICD + Mesp2
Specimen 3J: embryonic day 11.0; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). J' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3J' , 3J NICD + Mesp2 , 3J NICD
Specimen 3K: embryonic day 11.0; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). K' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3K NICD , 3K NICD + Mesp2 , 3K'
Specimen 3L: embryonic day 11.0; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Mesp2 (assay MGI:5805846). L' is a higher magnification of the double labeled panel.
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted 3L' , 3L NICD , 3L NICD + Mesp2
Specimen 5I,K: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5I , 5K
Specimen 5M,O: embryonic day 11.0; Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5O , 5M
Specimen 5Q,S: embryonic day 11.0; Ripply1tm1.1Sjt/Ripply1tm1.1Sjt, Ripply2tm1.1Ysa/Ripply2tm1.1Ysa (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present 5Q , 5S
Specimen S4 Wild-type: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S4_Wild-type Expression is in a narrow domain in the anterior presomitic mesoderm.
Specimen S4 Mesp2-null: embryonic day 11.0; Mesp2tm6Ysa/Mesp2tm6Ysa (more )
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S4_Mesp2-null Expression is in a broad domain in the anterior presomitic mesoderm.
Specimen S5A: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5A NICD + Tbx6 , S5A NICD
Specimen S5B: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5B NICD , S5B NICD + Tbx6
Specimen S5C: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5C NICD + Tbx6 , S5C NICD
Specimen S5D: embryonic day 11.0 (more )
Note: Double labeled: magenta - Notch1; green - Tbx6 (assay MGI:5805858).
Structure Level Pattern Image Note
TS18: tail unsegmented mesenchyme Present Regionally restricted S5D NICD + Tbx6 , S5D NICD
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory