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Gene Expression Data
Assay Details
Assay
Reference: J:63423 Kania A, et al., Coordinate roles for LIM homeobox genes in directing the dorsoventral trajectory of motor axons in the vertebrate limb. Cell. 2000 Jul 21;102(2):161-73
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5796151
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-NF
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2D: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present Regionally restricted 2D Expression in axons of dorsal root ganglion neurons.
TS19: segmental spinal nerve Present 2D
Specimen 2E: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 2E
Specimen 2F: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 2F
Specimen 2G: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 2G
Specimen 2H: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 2H
Specimen 4A: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: hindlimb bud Present 4A
Specimen 6B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: segmental spinal nerve Present Regionally restricted 6B Expression in motor nerves.
Specimen 6C: embryonic day 10.5 (more )
Note: Double labeled: red - Nefm; green - Lmx1b (assay MGI:5796154).
Structure Level Pattern Image Note
TS17: segmental spinal nerve Present 6C
Specimen 6D: embryonic day 11.5 (more )
Note: Double labeled: red - Nefm; green - Lmx1b (assay MGI:5796154).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 6D
Specimen 6E: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6E Expression in axonal projection of limb.
Specimen 6F: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6F Expression in axonal projection into hindlimb.
Specimen 6G: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+, Lmx1btm1Rjo/Lmx1btm1Rjo (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 6G
Specimen 6H: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+, Lmx1btm1Rjo/Lmx1btm1Rjo (more )
Note: Double labeled: red - Nefm; green - Lhx1 reporter (assay MGI:5796148).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present 6H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory