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Gene Expression Data
Assay Details
Assay
Reference: J:63423 Kania A, et al., Coordinate roles for LIM homeobox genes in directing the dorsoventral trajectory of motor axons in the vertebrate limb. Cell. 2000 Jul 21;102(2):161-73
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5796148
Gene symbol: Lhx1
Gene name: LIM homeobox protein 1
Reporter: lacZ
Antibody: anti-B-galactosidase
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1E: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Lhx1 protein (assay MGI:5796149) and Lhx5 (assay MGI:5796150).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1E In lumbar spinal cord, expression in dorsal and ventral interneurons.
Specimen 1G: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Lhx1 protein (assay MGI:5796149) and Lhx5 (assay MGI:5796150).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1G In right ventral quadrant of lumbar spinal cord, expression in LMC(I) neurons and ventral neurons.
Specimen 2C,2D: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 2C , 2D Expression in LMC neurons and axons in ventral root.
TS19: segmental spinal nerve Ambiguous 2D , 2C No expression in axons of dorsal root ganglion neurons.
Specimen 2E: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 2E Expression is dorsally restricted in motor axons at base of the hindlimb.
Specimen 2F: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 2F Expression is dorsally restricted in motor axons at base of the hindlimb.
Specimen 2G: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 2G At hindlimb level, expression in axons confined to the dorsal branch.
Specimen 2H: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 2H At hindlimb level, dorsally restricted expression.
Specimen 4A: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4A Expression in axons of lateral motor column, lateral division.
Specimen 6E: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6E Expression in lateral motor column, lateral division, axons with a dorsal trajectory.
Specimen 6F: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+ (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6F Expression in lateral motor column, lateral division, axons confined to dorsal nerve branch.
Specimen 6G: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+, Lmx1btm1Rjo/Lmx1btm1Rjo (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6G Expression in axons in dorsal and ventral regions of nascent motor nerve branches.
Specimen 6H: embryonic day 11.5; Lhx1tm1Tmj/Lhx1+, Lmx1btm1Rjo/Lmx1btm1Rjo (more )
Note: Double labeled: green - Lhx1 reporter; red - Nefm (assay MGI:5796151).
Structure Level Pattern Image Note
TS19: segmental spinal nerve Present Regionally restricted 6H Expression in axons in both dorsal and ventral nerve branches.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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