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Gene Expression Data
Assay Details
Assay
Reference: J:90073 Miyoshi G, et al., Identification of a novel basic helix-loop-helix gene, Heslike, and its role in GABAergic neurogenesis. J Neurosci. 2004 Apr 7;24(14):3672-82
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5780714
Gene symbol: Helt
Gene name: helt bHLH transcription factor
Antibody: Heslike antibody
Detection system: Secondary antibody
Results
Specimen 4A,B,D: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mki67 (assay MGI:5780715).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 4D , 4B , 4A Authors state that all expressing cells also express Mki67.
Specimen 4E,F,H: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - phospho-histone 3 (PH3).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 4E , 4H , 4F Authors state some expressing cells express phosphorylated histome H3.
Specimen 4I: embryonic day 9.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4I Authors report expression overlaps with the Nkx2-2 domain.
Specimen 4J: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 4J Expression is expanded and includes the Nkx2-2 domain.
Specimen 4K: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 4K Expression domain overlaps both domains of Nkx2-2 expression.
Specimen 4L: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 4L Expression domain is further expanded dorsally, nearly reaching the roof plate, and still overlaps both Nkx2-2 domains.
Specimen E13.5 not shown: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted Authors state expression is downregulated.
Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted Authors state expression disappears from the Nkx2.2 domain.
Specimen 5B: embryonic day 9.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 5B Lower levels of expression around the alar-basal boundary.
Specimen 5C: embryonic day 9.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 5C Many expressing cells coexpress Mash1 (Ascl1).
Specimen 5E: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS17: midbrain ventricular layer Present 5E/E' Expression in ventricular zone.
Specimen 5F: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS17: midbrain ventricular layer Present 5F/F' Expression in ventricular zone. Expressing cells also express Mash1 (Ascl1).
Specimen 5H: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5H/H' Dorsal expansion of expression domain.
Specimen 5I: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5I/I' Most expressing cells coexpress Mash1 (Ascl1).
Specimen 5K: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5K/K' Dorsal expansion of expression domain.
Specimen 5L: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5L/L' Most expressing cells coexpress Mash1 (Ascl1).
Specimen 6B: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6B Two stripes of expression in ventricular regions in the pretectum.
Specimen 6C: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6C Most expressing cells coexpress Mash1 (Ascl1).
Specimen 6D: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6D Region of expression overlaps with Nkx2-2 expression domains.
Specimen 6F: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS19: diencephalon ventricular layer Present Regionally restricted 6F Dorsal stripe of expression in ventricular region is expanded dorsally.
Specimen 6G: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6G Most expressing cells coexpress Mash1 (Ascl1).
Specimen 6H: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6H Some regions of expression overlap with Nkx2-2 expression domains.
Specimen 6J: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS20: diencephalon ventricular layer Present Regionally restricted 6J Dorsal stripe of expression in ventricular region is expanded dorsally.
Specimen 6K: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6K Most expressing cells coexpress Mash1 (Ascl1)
Specimen 6L: embryonic day 12.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Nkx2-2 (assay MGI:5780716).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6L Some regions of expression overlap with Nkx2-2 expression domains.
Specimen 6M: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718)
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6M Region of expression near the zona limitans intrathalamica.
Specimen 6N,O: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6N/O Coexpression in Mash1 (Ascl1) in a stripe just caudal to the zona limitans intrathalamica.
Specimen 6P: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6P Coexpression in Mash1 (Ascl1) in a stripe just caudal to the zona limitans intrathalamica.
Specimen 7A: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS17: midbrain Present 7A
Specimen 7E: embryonic day 10.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS17: midbrain Present 7E
Specimen 7I: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS19: midbrain Present 7I
Specimen 7M: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS19: midbrain Present 7M
Specimen 7Q: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: telencephalon Present 7Q
Specimen 7R: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS20: diencephalon Present 7R
Specimen 8A: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 8A/A' Expression in ventricular cells.
Specimen 8B: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Note: Double labeled: red - Heslike (Helt); green - Mash1 (Ascl1 assay MGI:5780719).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 8B/B' Increase in the number of expressing ventricular cells.
Specimen 8C: embryonic day 11.5 (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 8C/C' Expression in ventricular cells.
Specimen 8D: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Note: Double labeled: red - Heslike (Helt); green - GAD65 (Gad2 assay MGI:5780718).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 8D/D' Increase in the number of expressing ventricular cells.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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