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Gene Expression Data
Assay Details
Assay
Reference: J:90073 Miyoshi G, et al., Identification of a novel basic helix-loop-helix gene, Heslike, and its role in GABAergic neurogenesis. J Neurosci. 2004 Apr 7;24(14):3672-82
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5780716
Gene symbol: Nkx2-2
Gene name: NK2 homeobox 2
Antibody: 74.5A5
Detection system: Secondary antibody
Results
Specimen 4I: embryonic day 9.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4I Expression domain extends from the alar-basal boundary into the ventral region of the mesencephalon.
Specimen 4J: embryonic day 10.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 4J Expression domain is restricted to the alar-basal boundary region.
Specimen 4K: embryonic day 11.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 4K At approximately E10.5-E11.5, a new domain appears dorsally.
Specimen E13.5 not shown: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted
Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted
Specimen 4L: embryonic day 12.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 4L Two domains of expression.
Specimen 6D: embryonic day 10.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6D Expression domains overlap with Helt-expressing regions.
Specimen 6H: embryonic day 11.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6H Expression domains overlap with some Helt-expressing regions.
Specimen 6L: embryonic day 12.5 (more )
Note: Double labeled: green - Nkx2-2; red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6L Expression domains overlap with some Helt-expressing regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory