Probe:	AB63319 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong		2a	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS17: midbrain	Strong		2a	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS17: telencephalon	Strong		2a	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS17: branchial arch	Present		2a
TS17: forelimb bud	Present		2a
TS17: hindlimb bud	Present		2a

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong		2a'	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.
TS17: future spinal cord	Strong		2a'	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.
TS17: hindbrain	Strong		2a'	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.
TS17: midbrain	Strong		2a'	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong		2b	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS19: midbrain	Strong		2b	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS19: telencephalon	Strong		2b	Strong expression in cortical anlage of telencephalic vesicle, alar plate of midbrain, and nervous plate of rhombencephalon.
TS19: branchial arch	Present		2b
TS19: forelimb bud	Present		2b
TS19: hindlimb bud	Present		2b

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong		2b' (left)	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.
TS19: future spinal cord	Strong		2b' (left)	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong		2b' (right)	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.
TS19: midbrain	Strong		2b' (right)	Major expression along dorsal midline from diencephalon through midbrain-hindbrain region to spinal cord.

Structure	Level	Pattern	Image	Note
TS23: embryo	Present	Widespread	3a	Expression throughout entire neocortex.
TS23: dorsal root ganglion	Strong		3a	Primarily expressed in cortical plate, subplate and ventricular zone.
TS23: forebrain	Strong		3a	Primarily expressed in cortical plate, subplate and ventricular zone.
TS23: medulla oblongata	Strong		3a	Primarily expressed in cortical plate, subplate and ventricular zone.
TS23: nasal epithelium	Strong		3a	Primarily expressed in cortical plate, subplate and ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	3b , 3b'
TS23: cerebral cortex ventricular layer	Strong		3b , 3b'
TS23: cortical plate	Strong		3b , 3b'
TS23: cortical subplate	Strong		3b , 3b'
TS23: cerebral cortex subventricular zone	Weak		3b , 3b'

Structure	Level	Pattern	Image	Note
TS23: medulla oblongata	Strong	Regionally restricted	3c' , 3c	Strong expression in neurons and glial cells.

Structure	Level	Pattern	Image	Note
TS23: vertebral cartilage condensation	Present		3d , 3d'
TS23: dorsal root ganglion	Present		3d , 3d'

Structure	Level	Pattern	Image	Note
TS23: heart	Weak	Regionally restricted	3e	Authors report lower expression in smooth muscle of heart.

Structure	Level	Pattern	Image	Note
TS23: esophagus epithelium	Present		3f
TS23: lobar bronchus	Present		3f
TS23: lung	Present		3f
TS23: main bronchus	Present		3f

Structure	Level	Pattern	Image	Note
TS23: liver	Present		3g

Structure	Level	Pattern	Image	Note
TS23: early nephron	Present		3h
TS23: nephrogenic zone	Present		3h
TS23: renal cortex	Present		3h
TS23: metanephros	Present	Regionally restricted	3h	Widely distributed in developing secretory nephrons.
TS23: maturing glomerular tuft	Strong		3h
TS23: mesenchyme of metanephros	Weak		3h

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis pars intermedia	Present		3i , 3i'	Expression in intermediate lobe and posterior pituitary.
TS23: neurohypophysis pars nervosa	Present		3i , 3i'	Expression in intermediate lobe and posterior pituitary.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Strong		3j , 3j'

Structure	Level	Pattern	Image	Note
TS23: pancreatic duct	Present	Regionally restricted	3k' , 3k	Expression mainly localized in ductal epithelium.
TS23: pancreas mesenchyme	Weak		3k' , 3k

Structure	Level	Pattern	Image	Note
TS23: adrenal cortex	Strong	Regionally restricted	3l , 3l'	Expression in zona glomerulosa.
TS23: adrenal medulla	Weak		3l' , 3l

Structure	Level	Pattern	Image	Note
TS23: tongue muscle	Strong		3m

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Strong		3n

Structure	Level	Pattern	Image	Note
TS23: cartilage condensation	Present	Regionally restricted	3o	Expression in proliferating chondrocytes during endochondral ossification. Nearly negative signals in hypertrophic chondrocytes at this time.

Structure	Level	Pattern	Image	Note
TS23: cartilage condensation	Present	Regionally restricted	3p	Expression in proliferating chondrocytes and perichondrium during endochondral ossification.
TS23: perichondrium	Present	Regionally restricted	3p	Expression in proliferating chondrocytes and perichondrium during endochondral ossification.
TS23: skeletal muscle	Present		3p	Authors state that exprfession in chondrocytes and perichondrium was weaker compared to skeletal muscle.