Gene Expression Data
Assay Details
Assay
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Reference:
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J:42772
Motoyama J, et al., Organogenesis of the liver, thymus and spleen is affected in jumonji mutant mice. Mech Dev. 1997 Aug;66(1-2):27-37
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5763242
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Gene symbol:
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Jarid2
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Gene name:
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jumonji and AT-rich interaction domain containing 2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 1% formaldehyde, 0.2% glutaraldehyde and specimens were postfixed in 2% glutaraldehyde.
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Results
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Specimen
4E:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: liver |
Weak |
Regionally restricted |
4E
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Expression is in fibroblastic cells.
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TS21: blood |
Present |
Regionally restricted |
4E
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Expression is in large hematopoietic cells and small hematopoietic cells.
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TS21: blood vessel endothelium |
Present |
Regionally restricted |
4E
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Expression is in the liver.
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Specimen
4F:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2jumonji
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Age:
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embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Other - See Notes
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: liver |
Weak |
Regionally restricted |
4F
|
Expression is in fibroblastic cells.
|
TS21: blood |
Present |
Regionally restricted |
4F
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Expression is in large hematopoietic cells and small hematopoietic cells.
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TS21: blood vessel endothelium |
Present |
Regionally restricted |
4F
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Expression is in the liver.
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Specimen
6A:
embryonic day 13.5; Jarid2jumonji/Jarid2 +
(more )
Note: Double labeled: blue - Jarid2 reporter; brown - Ache (enzymatic activity).
Specimen
6A:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; brown - Ache (enzymatic activity).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: liver |
Present |
|
6A
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|
Specimen
6B:
embryonic day 13.5; Jarid2jumonji/Jarid2 +
(more )
Note: Double labeled: blue - Jarid2 reporter; brown - Selp (assay MGI:5763245).
Specimen
6B:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; brown - Selp (assay MGI:5763245).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: liver |
Present |
|
6B
|
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Specimen
7A:
embryonic day 13.5; Jarid2jumonji/Jarid2 +
(more )
Note: Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
Specimen
7A:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
|
Specimen
7B:
embryonic day 14.5; Jarid2jumonji/Jarid2 +
(more )
Note: Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
Specimen
7B:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
|
Specimen
7C:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2jumonji
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
|
Specimen
7D:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2jumonji
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Jarid2 reporter; purple - Ptprc (assay MGI:5763248).
|
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