Gene Expression Data
Assay Details
Assay
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Reference:
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J:42772
Motoyama J, et al., Organogenesis of the liver, thymus and spleen is affected in jumonji mutant mice. Mech Dev. 1997 Aug;66(1-2):27-37
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5763245
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Gene symbol:
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Selp
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Gene name:
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selectin, platelet
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Antibody:
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anti-P-selectin
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Assay notes:
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Fixative was 1% formaldehyde, 0.2% glutaraldehyde and specimens were postfixed in 2% glutaraldehyde.
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Results
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Specimen
6B:
embryonic day 13.5; Jarid2jumonji/Jarid2 +
(more )
Note: Double labeled: brown - Selp; blue - Jarid2 reporter (assay MGI:5763242).
Specimen
6B:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2+
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Age:
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embryonic day 13.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Selp; blue - Jarid2 reporter (assay MGI:5763242).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
6B
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Expression is in megakaryocytes.
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TS21: liver |
Present |
Single cells |
6B
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Expression is in megakaryocytes.
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Specimen
6C:
embryonic day 13.5
(more )
Specimen
6C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * BALB/cA
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
6C
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Expression is in megakaryocytes.
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TS21: liver |
Present |
Single cells |
6C
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Expression is in megakaryocytes.
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Specimen
6D:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2jumonji
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
6D
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Expression is in megakaryocytes in the liver and the number of positive cells is greater than in wild-type embryos.
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TS21: liver |
Present |
Single cells |
6D
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Expression is in megakaryocytes in the liver and the number of positive cells is greater than in wild-type embryos.
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Specimen
Table 3 E11.5 control:
embryonic day 11.5
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E11.5 control:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * BALB/cA
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Control was either +/+ or +/-.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: blood |
Present |
Single cells |
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Expression is in megakaryocytes in the liver.
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TS19: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
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Specimen
Table 3 E11.5 control +/-:
embryonic day 11.5; Jarid2jumonji/Jarid2 +
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E11.5 control +/-:
(close )
Genetic Background:
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C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
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TS19: liver |
Present |
Single cells |
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Expression is in megakaryocytes in the liver.
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Specimen
Table 3 E11.5 -/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2jumonji
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: blood |
Present |
Single cells |
|
Expression in megakaryocytes in the liver is the same as in wild-type.
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TS19: liver |
Present |
Single cells |
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Expression in megakaryocytes in the liver is the same as in wild-type.
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Specimen
Table 3 E12.5 control:
embryonic day 12.5
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E12.5 control:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/cA
|
Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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Other - See Notes
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Note:
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Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
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TS20: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
Specimen
Table 3 E12.5 control +/-:
embryonic day 12.5; Jarid2jumonji/Jarid2 +
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E12.5 control +/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
|
Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Note:
|
Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
TS20: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
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Specimen
Table 3 E12.5 -/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
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Jarid2jumonji/Jarid2jumonji
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: blood |
Present |
Single cells |
|
The number of megakaryocytes in the liver has increased in this mutant.
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TS20: liver |
Present |
Single cells |
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The number of megakaryocytes in the liver has increased in this mutant.
|
Specimen
Table 3 E13.5 control:
embryonic day 13.5
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E13.5 control:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/cA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Note:
|
Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
TS21: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
Specimen
Table 3 E13.5 control +/-:
embryonic day 13.5; Jarid2jumonji/Jarid2 +
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E13.5 control +/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
|
Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Note:
|
Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
TS21: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
Specimen
Table 3 E13.5 -/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
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Mutant Allele(s):
|
Jarid2jumonji/Jarid2jumonji
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood |
Present |
Single cells |
|
The number of megakaryocytes in the liver has increased in this mutant.
|
TS21: liver |
Present |
Single cells |
|
The number of megakaryocytes in the liver has increased in this mutant.
|
Specimen
Table 3 E14.5 control:
embryonic day 14.5
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E14.5 control:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/cA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Note:
|
Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
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TS22: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
Specimen
Table 3 E14.5 control +/-:
embryonic day 14.5; Jarid2jumonji/Jarid2 +
(more )
Note: Control was either +/+ or +/-.
Specimen
Table 3 E14.5 control +/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
|
Mutant Allele(s):
|
Jarid2jumonji/Jarid2+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Note:
|
Control was either +/+ or +/-.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: blood |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
TS22: liver |
Present |
Single cells |
|
Expression is in megakaryocytes in the liver.
|
Specimen
Table 3 E14.5 -/-:
(close )
Genetic Background:
|
C.129P2-Jarid2jumonji
|
Mutant Allele(s):
|
Jarid2jumonji/Jarid2jumonji
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: blood |
Present |
Single cells |
|
Expression in megakaryocytes in the liver is the same as in wild-type.
|
TS22: liver |
Present |
Single cells |
|
Expression in megakaryocytes in the liver is the same as in wild-type.
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