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Gene Expression Data
Assay Details
Assay
Reference: J:137140 Hald J, et al., Generation and characterization of Ptf1a antiserum and localization of Ptf1a in relation to Nkx6.1 and Pdx1 during the earliest stages of mouse pancreas development. J Histochem Cytochem. 2008 Jun;56(6):587-95
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5696625
Gene symbol: Ptf1a
Gene name: pancreas specific transcription factor, 1a
Antibody: anti-Ptf1a
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2A: postnatal adult (more )
Note: Double labeled: red - Ptf1a; green - Amy (assay MGI:5696771).
Structure Level Pattern Image Note
TS28: pancreatic acinus Present 2A
TS28: islets of Langerhans Absent 2A
Specimen 2D/E: embryonic day 15.5 (more )
Note: Double labeled: red - Ptf1a; green - Amy (assay MGI:5696771).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present 2E , 2D
TS23: duodenum Absent 2D
Specimen S1C: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: pancreas Present Regionally restricted S1C Expression is restricted to the exocrine pancreas; it is not present in the endocrine pancreas.
TS26: pancreatic acinus Present S1C
Specimen S1F: embryonic day 15.5 (more )
Note: Triple labeled: red - Ptf1a; green - Amy (assay MGI:5696771); blue - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present S1F
Specimen 2G: embryonic day 10.5 (more )
Note: Double labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 2G
TS17: pancreas primordium ventral bud Present 2G
TS17: neural tube Present 2G
Specimen 2H: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube Present 2H
Specimen 2I: embryonic day 10.5 (more )
Note: Double labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS17: neural tube Present 2I
Specimen 2J: embryonic day 10.5 (more )
Note: Double labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS17: neural tube Present 2J
TS17: pancreas primordium dorsal bud Present 2J
Specimen not shown: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: foregut-midgut junction Present Regionally restricted Expression is first seen in the ventral pancreas.
Specimen 3A: embryonic day 8.75 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS14: foregut-midgut junction Weak Regionally restricted 3A5 , 3A4 , 3A2 Weak expression is seen in the dorsal pancreas.
Specimen 3B: embryonic day 8.75 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS14: foregut-midgut junction Present Scattered 3B5 , 3B4 , 3B2 Scattered expression is seen in the ventral pancreas.
Specimen 3C: embryonic day 9.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Strong 3C2 , 3C5 , 3C4
TS15: pancreas primordium ventral bud Strong 3C2 , 3C5 , 3C4
Specimen 3D: embryonic day 10.0 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS16: pancreas primordium ventral bud Strong 3D2 , 3D5 , 3D4
TS16: pancreas primordium dorsal bud Present Non-Uniform 3D5 , 3D4 , 3D2 Expression is generally random, although clusters of cells are seen.
Specimen 3E: embryonic day 10.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong Uniform 3E2 , 3E4 , 3E5
TS17: pancreas primordium ventral bud Strong Uniform 3E2 , 3E4 , 3E5
Specimen 3F: embryonic day 11.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS19: pancreas primordium ventral bud Strong Non-Uniform 3F4 , 3F5 , 3F2 Expression is strong at the rim and weak in the center of the bud.
TS19: pancreas primordium dorsal bud Strong Non-Uniform 3F4 , 3F5 , 3F2 Expression is strong at the rim and absent in the center of the bud.
Specimen 3G: embryonic day 11.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Regionally restricted 3G4 , 3G5 , 3G2 Expression is strong at the rim but is extinguished in the very center of the bud. It is also present in the stalk.
Specimen 3H: embryonic day 12.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS20: pancreas Strong Regionally restricted 3H5 , 3H2 , 3H4 Expression is mainly seen in the tips of the branches.
Specimen 3I: embryonic day 12.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS20: pancreas Strong Regionally restricted 3I5 , 3I4 , 3I2 Expression is mainly seen in the tips of the branches.
Specimen 3J: embryonic day 12.5 (more )
Note: Triple labeled: red - Ptf1a; green - Nkx6-1 (assay MGI:5696770); gray - Pdx1 (assay MGI:5696775).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3J2 , 3J4 , 3J5 In the stalk region, expression is located at the rim.
TS20: duodenum Absent 3J4 , 3J2 , 3J5
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory