Antibody:	anti-Nkx6.1
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		S1F

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present		2G
TS17: neural tube	Present		2G

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		2I

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		2J
TS17: pancreas primordium dorsal bud	Present		2J

Structure	Level	Pattern	Image	Note
TS14: foregut-midgut junction	Absent		3A1 , 3A5 , 3A4	No expression is seen in the dorsal pancreas.

Structure	Level	Pattern	Image	Note
TS14: foregut-midgut junction	Present	Regionally restricted	3B4 , 3B5 , 3B1	Expression is seen in the ventral pancreas.

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium dorsal bud	Present		3C1 , 3C5 , 3C4
TS15: pancreas primordium ventral bud	Trace	Single cells	3C4 , 3C5 , 3C1	Weak expression is seen in only a few cells.

Structure	Level	Pattern	Image	Note
TS16: pancreas primordium dorsal bud	Present	Non-Uniform	3D1 , 3D5 , 3D4	Expression is generally random, although clusters of cells are seen.
TS16: pancreas primordium ventral bud	Trace		3D5 , 3D1 , 3D4	Expression is close to undetectable.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Present	Uniform	3E1 , 3E5 , 3E4
TS17: pancreas primordium ventral bud	Weak	Uniform	3E4 , 3E1 , 3E5

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium dorsal bud	Strong	Non-Uniform	3F1 , 3F5 , 3F4	Expression is strong in the center of the bud, weaker at the rim.
TS19: pancreas primordium ventral bud	Strong	Non-Uniform	3F1 , 3F5 , 3F4	Expression is strong in the center of the bud, weaker at the rim.

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium dorsal bud	Present	Regionally restricted	3G5 , 3G1 , 3G4	Expression is extinguished in the very center of the bud. It is present in the stalk.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Strong	Widespread	3H4 , 3H5 , 3H1	Expression is widespread, except for the tips. There it is seen in only a few, weakly expressing cells.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Strong	Widespread	3I5 , 3I1 , 3I4	Expression is widespread, except for the tips. There it is seen in only a few, weakly expressing cells.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Strong	Regionally restricted	3J1 , 3J4 , 3J5	In the stalk region, expression is strong in the center and weak at the rim.
TS20: duodenum	Absent		3J4 , 3J1 , 3J5