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Gene Expression Data
Assay Details
Assay
Reference: J:137140 Hald J, et al., Generation and characterization of Ptf1a antiserum and localization of Ptf1a in relation to Nkx6.1 and Pdx1 during the earliest stages of mouse pancreas development. J Histochem Cytochem. 2008 Jun;56(6):587-95
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5696775
Gene symbol: Pdx1
Gene name: pancreatic and duodenal homeobox 1
Antibody: anti-Pdx1
Detection system: Secondary antibody
Results
Specimen 3A: embryonic day 8.75 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS14: foregut-midgut junction Weak Regionally restricted 3A4 , 3A3 Expression is seen in the dorsal pancreas.
Specimen 3B: embryonic day 8.75 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS14: foregut-midgut junction Present Scattered 3B4 , 3B3 Scattered expression is seen in the ventral pancreas.
Specimen 3C: embryonic day 9.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present 3C4 , 3C3
TS15: pancreas primordium ventral bud Present 3C4 , 3C3
Specimen 3D: embryonic day 10.0 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS16: pancreas primordium dorsal bud Present Non-Uniform 3D4 , 3D3 Expression is generally random, although clusters of cells are seen.
TS16: pancreas primordium ventral bud Present Non-Uniform 3D4 , 3D3 Expression is generally random, although clusters of cells are seen.
Specimen 3E: embryonic day 10.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Uniform 3E4 , 3E3
TS17: pancreas primordium ventral bud Present Uniform 3E4 , 3E3
Specimen 3F: embryonic day 11.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong Uniform 3F3 , 3F4
TS19: pancreas primordium ventral bud Strong Uniform 3F3 , 3F4
Specimen 3G: embryonic day 11.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong Uniform 3G3 , 3G4 , 3G1 As well as being seen in the dorsal bud, expression is also seen in the stalk.
Specimen 3H: embryonic day 12.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS20: pancreas Present Widespread 3H4 , 3H3
Specimen 3I: embryonic day 12.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS20: pancreas Present Widespread 3I3 , 3I4
Specimen 3J: embryonic day 12.5 (more )
Note: Triple labeled: gray - Pdx1; red - Ptf1a (assay MGI:5696625); green - Nkx6-1 (assay MGI:5696770).
Structure Level Pattern Image Note
TS20: pancreas Present 3J3 , 3J4 Uniform expression is seen in the stalk.
TS20: duodenum Absent 3J3 , 3J4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory