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Gene Expression Data
Assay Details
Assay
Reference: J:202990 Lehigh KM, et al., Parcellation of the thalamus into distinct nuclei reflects EphA expression and function. Gene Expr Patterns. 2013 Dec;13(8):454-63
Assay type: RNA in situ
MGI Accession ID: MGI:5695630
Gene symbol: Epha4
Gene name: Eph receptor A4
Probe: EphA4 cDNA6
Probe preparation: labelled with radioactivity
Results
Specimen 1B/D: embryonic day 12.5 (more )
Note: Wild type mice were either CD-1 or C57BL/6. Pseudocolored and overlaid: red - Epha4; green - Epha7 (assay MGI:5696230).
Structure Level Pattern Image Note
TS20: epithalamus Present 1D , 1B There was some colocalization with EphA7.
TS20: thalamus Present 1D , 1B There was some colocalization with EphA7.
TS20: hypothalamus Present 1D , 1B
TS20: prethalamus Present 1D , 1B
TS20: prosomere 2 Present 1D , 1B
TS20: prosomere 3 Present 1D , 1B
Specimen 1F/I/J: embryonic day 14.5 (more )
Note: Wild type mice were either CD-1 or C57BL/6. Pseudocolored and overlaid in I,J: red - Epha4; green (I)/red (J) - Epha7 (assay MGI:5696230); green (J) - Efna5 (assay MGI:5696231).
Structure Level Pattern Image Note
TS22: thalamus Strong Regionally restricted 1J , 1F , 1I Expression was detected in a lateral-medial band within the dorsal thalamus. Expressing cells were largely distinct from cells expressing Efna5. Co-localization with Epha7 was noted in discrete portions of the positive expression domain.
Specimen 2B,E,F: embryonic day 15.5 (more )
Note: Wild type mice were either CD-1 or C57BL/6. Pseudocolored and overlaid in E,F: red - Epha4; green (E)/red (F) - Epha7 (assay MGI:5696230); green (F) - Efna5 (assay MGI:5696231).
Structure Level Pattern Image Note
TS23: thalamus Present Graded 2B , 2F , 2E Expression was detected in an anterior-high, posterior-low gradient. There was colocalization with Efna5 in the anterior region of the dorsal thalamus.
TS23: prethalamus Present Regionally restricted 2E , 2B , 2F Expression was detected in the dorsal prethalamus.
TS23: zona incerta Present 2E , 2B , 2F
Specimen 3B,E,F: embryonic day 17.5 (more )
Note: Wild type mice were either CD-1 or C57BL/6. Pseudocolored and overlaid in E,F: red - Epha4; green (E)/red (F) - Epha7 (assay MGI:5696230); green (F) - Efna5 (assay MGI:5696231).
Structure Level Pattern Image Note
TS25: thalamus Present Widespread 3E , 3F , 3B Expression was detected throughout the thalamus. There was overlap in the domains of Epha4 and Epha7.
TS25: dorsal lateral geniculate nucleus Strong 3F , 3B , 3E
TS25: posterior thalamic nucleus Present Non-Uniform 3F , 3B , 3E Expression was high in the lateral posterior and low in the ventral posterior nuclei. There was overlap with Efna5 in a lateral portion.
TS25: reticular thalamic nucleus Absent 3F , 3B , 3E
TS25: ventral lateral geniculate nucleus Absent 3F , 3B , 3E
TS25: zona incerta Absent 3F , 3B , 3E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory