Unc5d RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199400 van den Heuvel DM, et al., Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brain. PLoS One. 2013;8(2):e55828
Assay type:	RNA in situ
MGI Accession ID:	MGI:5693260
Gene symbol:	Unc5d
Gene name:	unc-5 netrin receptor D

Probe:	Unc5d cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2D ©

Specimen 2D: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: main olfactory bulb mitral cell layer	Present	2D
TS24: main olfactory bulb prospective granule cell layer	Weak	2D
TS24: olfactory epithelium	Weak	2D
TS24: main olfactory bulb ventricular layer	Strong	2D

2H ©

Specimen 2H: postnatal day 5 (more

)

Structure	Level	Image	Note
TS28: main olfactory bulb glomerular layer	Present	2H	Expression was reduced compared to E16.5.
TS28: main olfactory bulb granule cell layer	Present	2H	Expression was reduced compared to E16.5.
TS28: main olfactory bulb mitral cell layer	Present	2H	Expression was reduced compared to E16.5.

Specimen Table S2 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: accessory olfactory bulb	Moderate
TS24: main olfactory bulb internal plexiform layer	Moderate
TS24: main olfactory bulb prospective granule cell layer	Moderate
TS24: olfactory epithelium	Moderate
TS24: ethmoid bone cribriform plate	Absent
TS24: main olfactory bulb external plexiform layer	Weak
TS24: main olfactory bulb prospective glomerular layer	Weak
TS24: main olfactory bulb ventricular layer	Very strong
TS24: main olfactory bulb mitral cell layer	Strong

Specimen Table S2 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: ethmoid bone cribriform plate	Absent
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: olfactory epithelium	Absent
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate
TS28: anterior olfactory nucleus	Strong

Specimen Table S2 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb ventricular layer	Weak
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb granule cell layer	Absent
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: main olfactory bulb mitral cell layer	Moderate

5D ©

Specimen 5D: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong		5D
TS24: cerebral cortex ventricular layer	Present		5D

5H ©

Specimen 5H: postnatal day 5 (more

)

Structure	Level	Image
TS28: cortical layer I	Present	5H
TS28: cortical layer II	Present	5H
TS28: cortical layer III	Present	5H
TS28: cortical layer IV	Present	5H

Specimen Table S3 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Absent
TS24: cerebral cortex marginal layer	Absent
TS24: cortical plate	Absent
TS24: cortical subplate	Absent
TS24: telencephalon pia mater	Absent
TS24: cerebral cortex subventricular zone	Strong
TS24: cerebral cortex ventricular layer	Weak

Specimen Table S3 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer V	Absent
TS28: cortical layer VI	Absent

Specimen Table S3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer V	Absent
TS28: cortical layer VI	Absent

7D ©

Specimen 7D: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: dentate gyrus primordium	Present		7D
TS24: hippocampus	Present	Regionally restricted	7D	Expression was detected in the CA region.

7H ©

Specimen 7H: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7H
TS28: hippocampus CA3	Present		7H

Specimen Table S4 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: hippocampus	Moderate	Regionally restricted	There was strong expression in the subventricular zone. No expression was in the CA region.
TS24: dentate gyrus primordium	Moderate
TS24: entorhinal cortex	Moderate
TS24: hippocampus ventricular layer	Weak

Specimen Table S4 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Absent
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Strong
TS28: dentate gyrus	Weak
TS28: dentate gyrus granule cell layer	Weak
TS28: dentate gyrus molecular layer	Absent
TS28: dentate gyrus polymorphic layer	Moderate
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum lacunosum	Absent
TS28: hippocampus stratum radiatum	Absent
TS28: entorhinal cortex	Moderate

Specimen Table S4 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Weak
TS28: hippocampus CA2	Weak
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Weak
TS28: dentate gyrus granule cell layer	Weak
TS28: dentate gyrus polymorphic layer	Weak
TS28: dentate gyrus molecular layer	Absent
TS28: entorhinal cortex	Absent
TS28: hippocampus stratum lacunosum	Absent
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent

10D ©

Specimen 10D: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: lateral habenular nucleus	Present		10D

10H ©

Specimen 10H: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Present		10H

10L ©

Specimen 10L: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: habenula	Absent		10L

Specimen Table S5 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: midbrain	Moderate	Regionally restricted	Expression was detected in the substantia nigra and the ventral tegmental area.
TS24: interpeduncular nucleus	Strong
TS24: telencephalon septum	Strong
TS24: corpus striatum	Moderate
TS24: lateral habenular nucleus	Moderate
TS24: hypothalamus	Strong	Regionally restricted	Expression was strong in the lateral hypothalamus.
TS24: medial habenular nucleus	Absent
TS24: thalamus	Absent

Specimen Table S5 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: ventral tegmental area	Moderate
TS28: hypothalamus lateral zone	Weak
TS28: paraventricular thalamic nucleus	Weak
TS28: thalamus	Weak
TS28: corpus striatum	Absent
TS28: interpeduncular nucleus	Absent
TS28: medial habenular nucleus	Absent

Specimen Table S5 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Weak
TS28: corpus striatum	Absent
TS28: hypothalamus lateral zone	Absent
TS28: interpeduncular nucleus	Absent
TS28: lateral habenular nucleus	Absent
TS28: medial habenular nucleus	Absent
TS28: paraventricular thalamic nucleus	Absent
TS28: substantia nigra	Absent
TS28: thalamus	Absent
TS28: ventral tegmental area	Absent

13D ©

Specimen 13D: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Absent		13D

13H ©

Specimen 13H: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak		13H

Specimen Table S6 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum Purkinje cell cluster	Strong
TS24: deep cerebellar nucleus	Strong
TS24: cerebellum external granule cell layer	Absent
TS24: cerebellum ventricular layer	Absent

Specimen Table S6 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum internal granule cell layer	Absent

Specimen Table S6 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum granule cell layer	Absent
TS28: cerebellum molecular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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