Antibody:	p57kip2 antibody (M-20; sc-1039)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: metanephros visceral epithelium	Present		1B

Structure	Level	Pattern	Image	Note
TS22: metanephros visceral epithelium	Present		1D

Structure	Level	Pattern	Image	Note
TS22: metanephros visceral epithelium	Absent		1F

Structure	Level	Pattern	Image	Note
TS22: metanephros visceral epithelium	Absent		1H

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		2B

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		2D

Structure	Level	Pattern	Image	Note
TS28: thymus	Absent		2F

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present	Regionally restricted	2H	Expression was detected in upper crypt epithelium, but not in the lower regions of the crypts.

Structure	Level	Pattern	Image	Note
TS17: optic cup	Absent		3B

Structure	Level	Pattern	Image	Note
TS24: lens equatorial epithelium	Present		3D	Coexpression with p27 (Cdkn1b) was noted.

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present		3F	Coexpression with p27 (Cdkn1b) was noted.

Structure	Level	Pattern	Image	Note
TS28: adrenal cortex	Present		4B
TS28: adrenal medulla	Absent		4B

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	4D	Expression was detected in the spiral limbus.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted		Expression was detected mostly in the intermediate zone where postmitotic neurons migrate.
TS15: endocardial lining	Present

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted		Expression was detected mostly in the intermediate zone where postmitotic neurons migrate.
TS19: dorsal root ganglion	Present
TS19: endocardial lining	Present
TS19: lens vesicle	Present
TS19: liver	Weak

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted		Expression was detected mostly in the intermediate zone where postmitotic neurons migrate.
TS21: dorsal root ganglion	Present
TS21: endocardial tissue	Present
TS21: lens	Present
TS21: lung	Present
TS21: metanephros	Present
TS21: cochlea	Weak	Regionally restricted		Expression was weak in anterior cochlea.
TS21: liver	Weak
TS21: pancreas	Weak
TS21: adrenal gland	Present	Regionally restricted		Expression was detected in the cortex.

Structure	Level	Pattern	Image	Note
TS23: brain	Weak	Regionally restricted		Expression was detected mostly in the intermediate zone where postmitotic neurons migrate.
TS23: adrenal cortex	Present
TS23: dorsal root ganglion	Present
TS23: gut	Present
TS23: lens	Present
TS23: lung	Present
TS23: metanephros	Present
TS23: skeletal muscle	Present
TS23: cochlea	Present	Regionally restricted		Expression was in anterior cochlea.
TS23: cartilage	Present	Regionally restricted		Expression was predominantly in hypertrophic chondrocytes.
TS23: endocardial tissue	Weak
TS23: liver	Weak
TS23: pancreas	Weak

Structure	Level	Pattern	Image	Note
TS25: adrenal cortex	Present
TS25: gut	Present
TS25: lens	Present
TS25: liver	Present
TS25: lung	Present
TS25: metanephros	Present
TS25: skin	Present
TS25: cartilage	Present	Regionally restricted		Expression was predominantly in hypertrophic chondrocytes.
TS25: secondary palate	Weak

Structure	Level	Pattern	Image	Note
TS27: adrenal cortex	Present
TS27: metanephros	Present

Structure	Level	Pattern	Image	Note
TS28: adrenal cortex	Present
TS28: metanephros	Present