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Gene Expression Data
Assay Details
Assay
Reference: J:154912 Suzuki H, et al., The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009 Dec 15;336(2):222-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5645471
Gene symbol: Nanos3
Gene name: nanos C2HC-type zinc finger 3
Antibody: Nanos3 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1H: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 1H Detected as single, 2, 4, 8, 16, or many cells connected via intercellular bridges.
Specimen 1I: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 1I Detected as single, 2, 4, 8, 16, or many cells connected via intercellular bridges.
Specimen 1J: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 1J Detected as single, 2, 4, 8, 16, or many cells connected via intercellular bridges.
Specimen 1K: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 1K Detected as single, 2, 4, 8, 16, or many cells connected via intercellular bridges.
Specimen 3A: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present 3A Merge , 3A upper 60.4% coexpressed Gfra1.
Specimen 3B: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present 3B Merge , 3B upper 60.4% coexpressed Gfra1.
Specimen 4A: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 4A
Specimen 4B: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule epithelium Present Clusters 4B Merge , 4B left Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia.
TS28: spermatogonium Present Clusters 4B left , 4B Merge Expressed in undifferentiated type-A spermatogonia (As to Aal). Most of them did not co-express Kit.
Specimen 4C: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule epithelium Present Clusters 4C left , 4C Merge Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia.
TS28: spermatogonium Present Clusters 4C Merge , 4C left Co-expressed with Kit in Aal spermatogonia.
Specimen 4D: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule epithelium Present Clusters 4D left , 4D Merge Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia.
TS28: spermatogonium Present Clusters 4D left , 4D Merge Weaker expression in A2 spermatogonia at stage IX.
Specimen 4E: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule epithelium Present Clusters 4E Merge , 4E left Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia.
TS28: spermatogonium Present Clusters 4E Merge , 4E left Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia. No expression in other spermatogonia during stages I-IV.
Specimen 4F: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Kit (assay MGI:5645664).
Structure Level Pattern Image Note
TS28: seminiferous tubule epithelium Present Clusters 4F Merge , 4F left Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia.
TS28: spermatogonium Present Clusters 4F Merge , 4F left Expressed in Kit-negative undifferentiated As, Apr and Aal spermatogonia. No expression in other spermatogonia during stages I-IV.
Specimen S2C: embryonic day 8.5; Nanos2tm2Ysa/Nanos2+ (more )
Note: Double labeled: red - Nanos3; green - Pou5f1 (assay MGI:5645478).
Structure Level Pattern Image Note
TS13: primordial germ cell Present S2C left , S2C merge Coexpressed with Pou5f1.
Specimen S2D: embryonic day 8.5; Nanos2tm2Ysa/Nanos2+ (more )
Note: Double labeled: red - Nanos3; green - Pou5f1 (assay MGI:5645478).
Structure Level Pattern Image Note
TS13: primordial germ cell Present S2D merge , S2D left Coexpressed with Pou5f1.
Specimen S6C: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S6C left , S6C Merge Expressed in Apr-like cell clusters.
Specimen S6D: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S6D left , S6D Merge Expressed in Aal-like cell clusters.
Specimen S6E: postnatal adult (more )
Note: Double labeled: red - Nanos3; green - Gfra1 (assay MGI:5645538).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S6E left , S6E Merge Expressed in Aal-like cell clusters.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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