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Gene Expression Data
Assay Details
Assay
Reference: J:154912 Suzuki H, et al., The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009 Dec 15;336(2):222-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5645478
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S2C: embryonic day 8.5; Nanos3tm3.1Ysa/Nanos3+ (more )
Note: Double labeled: green - Pou5f1; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS13: primordial germ cell Present S2C middle , S2C merge
Specimen S2D: embryonic day 8.5; Nanos3tm3.1Ysa/Nanos3+ (more )
Note: Double labeled: green - Pou5f1; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS13: primordial germ cell Present S2D merge , S2D middle
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory