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Gene Expression Data
Assay Details
Assay
Reference: J:154912 Suzuki H, et al., The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009 Dec 15;336(2):222-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5645664
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-CD117 (ACK2)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Clusters 4A Expressed in a portion of the Aal and differentiating spermatogonia.
Specimen 4B: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters 4B Merge , 4B middle No expression in most of the Nanos3+ undifferentiated type-A spermatogonia (As to Aal).
Specimen 4C: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: spermatogonium Strong Clusters 4C Merge , 4C middle Co-expressed with Nanos3 in Aal spermatogonia at around stage VII.
Specimen 4D: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters 4D middle , 4D Merge
Specimen 4E: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters 4E Merge , 4E middle
Specimen 4F: postnatal adult (more )
Note: Double labeled: green - Kit; red - Nanos3 (assay MGI:5645471).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters 4F middle , 4F Merge
Specimen S4C: postnatal adult (more )
Note: Double labeled: green - Kit; red - Zbtb16 (assay MGI:5645701).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S4C middle , S4C Merge Co-expressed with Zbtb16 in Aal and a portion of the differentiating spermatogonia.
Specimen S4D: postnatal adult (more )
Note: Double labeled: green - Kit; red - Zbtb16 (assay MGI:5645701).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S4D middle , S4D Merge Co-expressed with Zbtb16 in Aal and a portion of the differentiating spermatogonia.
Specimen S4E: postnatal adult (more )
Note: Double labeled: green - Kit; red - Zbtb16 (assay MGI:5645701).
Structure Level Pattern Image Note
TS28: spermatogonium Present Clusters S4E Merge , S4E middle Expressed in B-type spermatogonia and preleptotene spermatocytes, which idd not express Zbtb16 at stages V-VIII.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory