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Gene Expression Data
Assay Details
Assay
Reference: J:106421 Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
Assay type: RNA in situ
MGI Accession ID: MGI:5632132
Gene symbol: Zfhx2os
Gene name: zinc finger homeobox 2, opposite strand
Probe: zfh-5os cDNA1
Probe preparation: labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors did not specify which probe was used. Either this probe or the opposite strands of zfh-5 cDNA2 MGI:5632099, zfh-5 cDNA3 MGI:5632100, or zfh-5 cDNA4 MGI:5632101 were used.
Results
Specimen 1B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex intermediate zone Present 1B Antisense
TS21: cerebral cortex subventricular zone Present 1B Antisense
TS21: ganglionic eminence Present 1B Antisense
TS21: cerebellum Present 1B Antisense Expression is in the deep cerebellar nuclei.
Specimen 2A E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: hippocampus Present 2A E16.5 AS
Specimen 2A P2: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: hippocampus Present 2A P2 AS
Specimen 2A P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: hippocampus Weak 2A P7 AS
TS28: dentate gyrus Present 2A P7 AS
Specimen 2A P20: postnatal day 20 (more )
Structure Level Pattern Image Note
TS28: hippocampus pyramidal cell layer Trace 2A P20 AS
TS28: dentate gyrus granule cell layer Weak 2A P20 AS
Specimen 2B: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: rostral migratory stream Present 2B Antisense
Specimen 2C left: postnatal adult (more )
Structure Level Pattern Image Note
TS28: dentate gyrus layer Present Regionally restricted 2C AS left Expression is in the subgranular zone.
Specimen 2C right: postnatal adult (more )
Structure Level Pattern Image Note
TS28: dentate gyrus layer Present Regionally restricted 2C AS right Expression is in the subgranular zone.
Specimen 2D a: postnatal adult (more )
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 2D aAS Expression is in migrating neurons along the rostral migratory stream to the olfactory bulbs.
Specimen 2D b: postnatal adult (more )
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 2D bAS Expression is in migrating neurons along the rostral migratory stream to the olfactory bulbs.
Specimen 2D c: postnatal adult (more )
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 2D cAS Expression is in migrating neurons along the rostral migratory stream to the olfactory bulbs.
Specimen 5A +/+ left: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex subventricular zone Present Regionally restricted 5A b , 5A Antisense +/+ Expression is in the basal part.
TS24: cerebral cortex ventricular layer Present Regionally restricted 5A b , 5A Antisense +/+ Expression is in the basal part.
TS24: cortical plate Present 5A Antisense +/+ , 5A b
Specimen 5A +/+ right: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pons Present Regionally restricted 5A Antisense right panel +/+ Expression is in pontine nuclei.
Specimen 5A deltaASC/deltaASC left: embryonic day 16.5; Zfhx2ostm1Ymri/Zfhx2ostm1Ymri (more )
Structure Level Pattern Image Note
TS24: brain Absent 5A Antisense mutant
Specimen 5A deltaASC/deltaASC right: embryonic day 16.5; Zfhx2ostm1Ymri/Zfhx2ostm1Ymri (more )
Structure Level Pattern Image Note
TS24: brain Absent 5A Antisense right panel mutant
Specimen 5B +/+: postnatal day 8 (more )
Structure Level Pattern Image Note
TS28: cerebellum granule cell layer Strong 5B Antisense +/+
Specimen 5B mutant: postnatal day 8; Zfhx2ostm1Ymri/Zfhx2ostm1Ymri (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent 5B Antisense mutant
Specimen 6B left: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex Present 6B left
Specimen 6B right: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: brain Present 6B right
TS24: pons Present Regionally restricted 6B right Expression is in pontine nuclei.
Specimen 6F left: embryonic day 16.5; Zfhx2/Zfhx2ostm2Ymri/Zfhx2+, Zfhx2/Zfhx2ostm2Ymri/Zfhx2os+ (more )
Note: This embryo was a single heterozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double heterozygote).
Structure Level Pattern Image Note
TS24: cerebral cortex Weak 6F left
Specimen 6F right: embryonic day 16.5; Zfhx2/Zfhx2ostm2Ymri/Zfhx2+, Zfhx2/Zfhx2ostm2Ymri/Zfhx2os+ (more )
Note: This animal is a single heterozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double heterozygote).
Structure Level Pattern Image Note
TS24: brain Weak 6F right
TS24: pons Weak Regionally restricted 6F right Expression is in pontine nuclei.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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