Zfhx2os^tm1Ymri
Targeted Allele Detail

Summary

• Symbol: Zfhx2os^tm1Ymri
• Name: zinc finger homeobox 2, opposite strand; targeted mutation 1, Tetsuo Yamamori
• MGI ID: MGI:3626364
• Synonyms: DeltaASC, Zfhx2/Zfhx2as^tm1Ymri
• Gene: Zfhx2os Location: Chr14:55310548-55313331 bp, + strand Genetic Position: Chr14, 28.06 cM
• Alliance: Zfhx2os^tm1Ymri page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106421
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: A targeting vector was designed to remove a 6 kb region containing the antisense control region, expecting that the transcription of the antisense RNA would be disrupted without altering the protein coding sequence of the sense RNA. The ASC was replaced with a neomycin resistance cassette. In situ hybridization of mutant E16.5 brains revealed that expression of Zfhx2os was under the detection limit, whereas that of Zfhx2 was increased in various areas of the brain, including ectopic expression in the pontine nuclei, where wild-type expresses Zfhx2os and not Zfhx2. Northern blot detected transcript from Zfhx2 but not Zfhx2os. (J:106421)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Zfhx2os Mutation: 11 strains or lines available

References

• Original: J:106421 Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
• All: 2 reference(s)