Zfhx2/Zfhx2os^tm2Ymri
Targeted Allele Detail

Summary

• Symbol: Zfhx2/Zfhx2os^tm2Ymri
• Name: zinc finger homeobox 2; targeted mutation 2, Tetsuo Yamamori
• MGI ID: MGI:3626365
• Synonyms: Zfh-5^EGFP, Zfhx2/Zfhx2as^tm2Ymri
• Gene: Zfhx2 Location: Chr14:55297719-55329781 bp, - strand Genetic Position: Chr14, 28.05 cM
• Alliance: Zfhx2/Zfhx2os^tm2Ymri page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106421
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A targeting vector was designed to replace exons 2-6 of Zfhx2 with that of EGFP-pA-neo, such that no functional Zfhx2 mRNA would be produced, but EGFP would be directed by the Zfhx2 promoter. Neither sense (Zfhx2) nor antisense (Zfhx2os) transcripts were expressed except for antisense exons 7-10. In situ hybridization detected ectopic expression of EGFP in the cerebral cortex and pontine nuclei, unlike wild-type expression of Zfhx2. Due to an incomplete termination at the polyA site of the neo gene, EGFP antisense transcripts were also detectable, however, at a much lower level than the wild-type antisense RNA. (J:106421)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Zfhx2 Mutation: 245 strains or lines available

References

• Original: J:106421 Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
• All: 2 reference(s)