Nodal RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:187958 Yoshiba S, et al., Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2. Science. 2012 Oct 12;338(6104):226-31
Assay type:	RNA in situ
MGI Accession ID:	MGI:5614332
Gene symbol:	Nodal
Gene name:	nodal growth differentiation factor

Probe:	Nodal probe16
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen 1C Pkd2+/+: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	1C Nodal +/+	Expression is in the left-side lateral plate mesoderm.

Specimen 1C Pkd2-/-: embryonic day 8.0; Pkd2^tm1Dwo/Pkd2^tm1Dwo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		1C Nodal -/-	Expression in the left-side lateral plate mesoderm is lost in this mutant.

Specimen S4A +/+: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	S4A Nodal +/+	There is a slight assymetry in crown cell expression, the right side is lower than left.

Specimen S4A -/-: embryonic day 8.0; Pkd2^tm1Dwo/Pkd2^tm1Dwo (more

)

Structure	Level	Pattern	Image	Note
TS12: node	Present	Regionally restricted	S4A Nodal -/-	There is bilateral equal expression in crown cells in this mutant.

Specimen S4B +/+: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		S4B top left

Specimen S4B Pkd2-/-: embryonic day 8.0; Pkd2^tm1Dwo/Pkd2^tm1Dwo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		S4B bottom left

Specimen S4B Cerl2-/- top: embryonic day 8.0; Dand5^tm1Belo/Dand5^tm1Belo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	S4B top middle	Left-sided expression in this mutant.

Specimen S4B Cerl2-/- bottom: embryonic day 8.0; Dand5^tm1Belo/Dand5^tm1Belo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		S4B bottom middle	Expression is in both sides in this mutant.

Specimen S4B Pkd2-/-;Cerl2/-/ top: embryonic day 8.0; Dand5^tm1Belo/Dand5^tm1Belo, Pkd2^tm1Dwo/Pkd2^tm1Dwo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	S4B top right	Expression was either left-sided (2 of 11), right-sided (3 of 11), bilateral (2 of 11), or absent (4 of 11) in Pkd2-/-;Cerl2-/- embryos.

Specimen S4B Pkd2-/-;Cerl2-/- bottom: embryonic day 8.0; Dand5^tm1Belo/Dand5^tm1Belo, Pkd2^tm1Dwo/Pkd2^tm1Dwo (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	S4B bottom right	Expression was either left-sided (2 of 11), right-sided (3 of 11), bilateral (2 of 11), or absent (4 of 11) in Pkd2-/-;Cerl2-/- embryos.

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