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Gene Expression Data
Assay Details
Assay
Reference: J:199568 Neufeld SJ, et al., mRNA fluorescence in situ hybridization to determine overlapping gene expression in whole-mount mouse embryos. Dev Dyn. 2013 Sep;242(9):1094-100
Assay type: RNA in situ
MGI Accession ID: MGI:5588380
Gene symbol: Hoxd13
Gene name: homeobox D13
Probe: Hoxd13 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Fluorescein
Assay notes: Tyramide Signal Amplification was used.
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Inset shows close-up view of the forelimb bud.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 1A Expression is in the posterior region.
TS17: hindlimb bud Present Regionally restricted 1A Expression is in the posterior region.
TS17: tail Present 1A
Specimen 1B: embryonic day 11.5 (more )
Note: Inset shows close-up view of the forelimb bud.
Structure Level Pattern Image Note
TS19: forelimb bud Present Regionally restricted 1B Expression is in the posterior region.
TS19: hindlimb bud Present Regionally restricted 1B Expression is in the posterior region.
TS19: genitourinary system Present Regionally restricted 1B Expression is in the genital bud.
Specimen 1C: embryonic day 12.5 (more )
Note: Inset shows close-up view of the forelimb bud.
Structure Level Pattern Image Note
TS20: foot Present 1C
TS20: hand Present 1C
Specimen 1D: embryonic day 13.5 (more )
Note: Inset shows close-up view of the forelimb bud.
Structure Level Pattern Image Note
TS21: foot Present 1D
TS21: hand Present 1D
Specimen 2B: embryonic day 10.5 (more )
Note: Double labeled: green - Hoxd13; red - Shh (assay MGI:5588436).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2C , 2B Expression is in the posterior region.
TS17: hindlimb bud Present Regionally restricted 2C , 2B Expression is in the posterior region.
Specimen 2E: embryonic day 10.5 (more )
Note: Double labeled: green - Hoxd13; red - Shh (assay MGI:5588436).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2F , 2E Expression is in the posterior region.
Specimen 2H: embryonic day 10.5 (more )
Note: Confocal image. Double labeled: green - Hoxd13; red - Shh (assay MGI:5588436).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2I , 2H Expression is in the posterior region.
Specimen 2K: embryonic day 10.5 (more )
Note: Double labeled: green - Hoxd13; red - Shh (assay MGI:5588436).
Structure Level Pattern Image Note
TS17: hindgut Present Regionally restricted 2L , 2K Expression is in the posterior hindgut endoderm.
Specimen 2N: embryonic day 10.5 (more )
Note: Confocal image. Double labeled: green - Hoxd13; red - Shh (assay MGI:5588436).
Structure Level Pattern Image Note
TS17: hindgut Present Regionally restricted 2O , 2N Expression is in the posterior hindgut endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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