Probe:	Sulf1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Present		1A
TS15: somite	Present		1A
TS15: telencephalon	Present		1A
TS15: tail	Present	Regionally restricted	1A	Expression is at the tip.
TS15: 1st branchial arch ectoderm	Present	Regionally restricted	1A	Expression is in the rostral surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		1B
TS17: nasal pit	Present		1B
TS17: oral epithelium	Present		1B
TS17: somite	Present		1B
TS17: telencephalon	Present		1B
TS17: tail	Present	Regionally restricted	1B	Expression is at the tip.
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	1B	Expression is in the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		1C
TS19: nasal pit	Present		1C
TS19: somite	Present		1C
TS19: telencephalon	Present		1C
TS19: tail	Present	Regionally restricted	1C	Expression is at the tip.
TS19: limb mesenchyme	Present	Regionally restricted	1C	Expression is in the distal mesenchyme, absent in proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	1H	Expression is in the distal mesenchyme.
TS17: floor plate	Present		1H

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		1K

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	1L	Expression is in the distal mesenchyme, absent in proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	1M	Expression is in the distal mesenchyme, absent in proximal mesenchyme.
TS20: femur pre-cartilage condensation	Absent		1M

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	1O	Expression is in synovial joints.
TS20: femur pre-cartilage condensation	Absent		1O

Structure	Level	Pattern	Image	Note
TS22: foot joint	Present		1P	Expression is in synovial joints.

Structure	Level	Pattern	Image	Note
TS22: elbow joint primordium	Present		2A
TS22: forelimb digit	Present		2A
TS22: forelimb cartilage condensation	Present	Regionally restricted	2A	Expression is in hypertrophic chondrocytes.
TS22: hand joint	Present	Regionally restricted	2A	Expression is in synovial joints.

Structure	Level	Pattern	Image	Note
TS24: forelimb digit	Present		2C
TS24: forelimb mesenchyme	Present	Regionally restricted	2C	Expression is in hypertrophic chondrocytes.
TS24: forelimb skeleton	Weak		2C	Absent in the perichodrium and present in the neighboring connective tissue, weak in bone.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Regionally restricted	2E	Expression is in fibrocartilaginous joints between individual sternebrae and between the sternum and ribs.

Structure	Level	Pattern	Image	Note
TS24: forelimb mesenchyme	Present	Regionally restricted	2G	xpression is in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Weak		2I

Structure	Level	Pattern	Image	Note
TS24: mandible	Weak		2K
TS24: lower jaw molar enamel organ	Present	Regionally restricted	2K	Expression is in ameloblasts.