Probe:	Sox10 probe9
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	1a	Expression was detected in migrating neural crest cells in orderly streams in the anterior trunk and in cranial neural crest cells.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	1b	Expression was detected in migrating neural crest cells emerging from the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	1c	Expression was detected in migrating neural crest cells. The trunk neural crest cells are random and disorderly in the mutant. In contrast, the cranial neural crest cells looked comparatively normal.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	1d	Reduced amounts of migrating neural crest cells were emerging from the dorsal neural tube in the mutant. An increased number of cells crossing the midline were noted in the mutant.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2b , 2a	Rostrally, expression in neural crest cells in somites were aggregating at future dorsal root ganglia and sypathetic ganglia. Caudally, expression was in neural crest cells migrating in orderly streams through the rostral half of the somites.
TS15: trunk somite	Present	Regionally restricted	2b , 2a	Rostrally, expression in neural crest cells in somites were aggregating at future dorsal root ganglia and sypathetic ganglia. Caudally, expression was in neural crest cells migrating in orderly streams through the rostral half of the somites.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2c	Expression was detected exclusively in neural crest cells migrating in the rostral half of the somites.
TS15: trunk somite	Present	Regionally restricted	2c	Expression was detected exclusively in neural crest cells migrating in the rostral half of the somites.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		2d
TS17: spinal nerve	Present		2d
TS17: sympathetic nervous system	Present	Regionally restricted	2d	Expression was detected in sympathetic chain ganglia.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2e , 2f	Expression was detected in neural crest cells migrating in a dispersed fashion through both halves of the somites.
TS15: trunk somite	Present	Regionally restricted	2e , 2f	Expression was detected in neural crest cells migrating in a dispersed fashion through both halves of the somites.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Regionally restricted	2g	Expression was detected in neural crest cells in the rostral and caudal halves of the somites. Fewer neural crest cells at the presumptive borders between somites maintained a pseudosegmental quality to the migration pattern in the mutant.
TS15: trunk somite	Present	Regionally restricted	2g	Expression was detected in neural crest cells in the rostral and caudal halves of the somites. Fewer neural crest cells at the presumptive borders between somites maintained a pseudosegmental quality to the migration pattern in the mutant.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		2h	The number of stained cells was reduced in the mutant.
TS17: spinal nerve	Present		2h	The number of stained cells was reduced in the mutant.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		3a

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		3b , 3c
TS17: migrating neural crest	Present	Regionally restricted	3b	Expression was detected in vagal neural crest cells migrating into the heart and gut.
TS17: sympathetic nervous system	Present	Regionally restricted	3b	Expression was detected in the cervical ganglia.

Structure	Level	Pattern	Image	Note
TS18: peripheral nervous system	Present	Regionally restricted	3d	Expression was detected in peripheral nerves.
TS18: cranial ganglion	Present	Regionally restricted	3d	Expression was detected in Schwann and satellite cell precursors.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		3e	Expression was detected as in wild type.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		3g , 3f	Expression was detected in the cranial ganglia, but phenotypic abnormalities were described.
TS17: migrating neural crest	Present	Regionally restricted	3f	Expression was detected in vagal neural crest cells migrating into the heart and gut, but the number cells appeared significantly reduced in the mutant.
TS17: sympathetic nervous system	Present	Regionally restricted	3f	A significant reduction in staining was noted in the cervical ganglia of the mutant.

Structure	Level	Pattern	Image	Note
TS18: peripheral nervous system	Present	Regionally restricted	3h	Expression was detected in peripheral nerves, but phenotypic abnormalities were noted.
TS18: cranial ganglion	Present	Regionally restricted	3h	Expression was detected in Schwann and satellite cell precursors, but phenotypic abnormalities were noted.