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Gene Expression Data
Assay Details
Assay
Reference: J:38196 Masuya H, et al., Multigenic control of the localization of the zone of polarizing activity in limb morphogenesis in the mouse. Dev Biol. 1997 Feb 1;182(1):42-51
Assay type: RNA in situ
MGI Accession ID: MGI:5491009
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 1B Expressed in the posterior margin.
Specimen 1E: embryonic day 12.5; Alx4lst/Alx4lst (more )
Note: The embryos were selected by their phenotype (preaxial polydactyly). It is possible that the genotype could be a heterozygous mutant.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 1E Expressed in the posterior margin. Ectopic expression in the mesenchyme at the anterior margin.
Specimen 1H: embryonic day 12.5; Lx/Lx (more )
Note: The embryos were selected by their phenotype (preaxial polydactyly). It is possible that the genotype could be a heterozygous mutant.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 1H Expressed in the posterior margin. Ectopic expression in the mesenchyme at the anterior margin.
Specimen 1K: embryonic day 12.5; Xpl/Xpl+ (more )
Note: The embryos were selected by their phenotype (preaxial polydactyly). It is possible that the genotype could be either a hemizygous male mutant or a homozygous female mutant.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 1K Expressed in the posterior margin. Ectopic expression in the mesenchyme at the anterior margin.
Specimen 2B: embryonic day 12.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS20: limb mesenchyme Present Regionally restricted 2B Expressed in the posterior margin. Ectopic expression in the mesenchyme at the anterior margin.
Specimen 3F: embryonic day 10.5 (more )
Note: Stage 1 forelimb.
Structure Level Pattern Image Note
TS17: forelimb bud Absent 3F
Specimen 3G: embryonic day 11.0 (more )
Note: Stage 2 forelimb.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present Regionally restricted 3G Expressed in the mesenchyme of the posterior margin.
Specimen 3H: embryonic day 11.5 (more )
Note: Stage 3 forelimb.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 3H Expressed in the mesenchyme of the posterior margin.
Specimen 3I: embryonic day 12.5 (more )
Note: Stage 5 forelimb.
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Strong Regionally restricted 3I Expressed in the mesenchyme of the posterior margin.
Specimen 3J: embryonic day 13.0 (more )
Note: Stage 8 forelimb.
Structure Level Pattern Image Note
TS21: forelimb Ambiguous 3J
Specimen 3O: embryonic day 10.5 (more )
Note: Stage 0 hindlimb.
Structure Level Pattern Image Note
TS17: hindlimb bud Absent 3O
Specimen 3P: embryonic day 11.0 (more )
Note: Stage 2 hindlimb.
Structure Level Pattern Image Note
TS18: hindlimb bud mesenchyme Present Regionally restricted 3P Expressed in the mesenchyme of the posterior margin.
Specimen 3Q: embryonic day 11.5 (more )
Note: Stage 4 hindlimb.
Structure Level Pattern Image Note
TS19: hindlimb bud mesenchyme Strong Regionally restricted 3Q Expressed in the mesenchyme of the posterior margin.
Specimen 3R: embryonic day 12.5 (more )
Note: Stage 7 hindlimb.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 3R Expression faded toward the posterior margin.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory