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Gene Expression Data
Assay Details
Assay
Reference: J:33053 Hug BA, et al., Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3. Mol Cell Biol. 1996 Jun;16(6):2906-12
Assay type: RT-PCR
MGI Accession ID: MGI:5484655
Gene symbol: Hbb-bs
Gene name: hemoglobin, beta adult s chain
Probe: Hbb-b-pA, Hbb-b-pB
Probe preparation: DNA
Visualized with: Phosphorimaging
Assay notes: Primers span intron 2 of both adult b-globin genes.
Results Image: 3
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
C57BL/6 x 129/SvJ (WT) E14.5 (a) TS22: liver Present Not Specified; total RNA (129/Sv x C57BL/6)F1 Not Specified
C57BL/6 x 129/SvJ ([d]HS3/NEO+) E14.5 (a) TS22: liver Present (b) Not Specified; total RNA involves: 129X1/SvJ * C57BL/6 Rr320tm1Ley/Rr320+Not Specified
C57BL/6 x 129/SvJ ([d]HS3/NEO-) E14.5 (a) TS22: liver Present (c) Not Specified; total RNA involves: 129X1/SvJ * C57BL/6 Rr320tm1.1Ley/Rr320+Not Specified
C57BL/6 x 129/SvJ (WT) P adult TS28: blood Present Not Specified; total RNA (129/Sv x C57BL/6)F1 Not Specified
C57BL/6 x 129/SvJ ([d]HS3/NEO+) P adult TS28: blood Present (d) Not Specified; total RNA involves: 129X1/SvJ * C57BL/6 Rr320tm1Ley/Rr320+Not Specified
C57BL/6 x 129/SvJ ([d]HS3/NEO-) P adult TS28: blood Present (e) Not Specified; total RNA involves: 129X1/SvJ * C57BL/6 Rr320tm1.1Ley/Rr320+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The [d]HS3/Neo2+ mutation reduces adult globin gene expression to 30% of the wild-type level.
(c) The [d]HS3/Neo2- mutation reduces adult globin gene expression to 74% of the wild-type level.
(d) The [d]HS3/Neo2+ mutation reduces adult globin gene expression to 44% of the wild-type level.
(e) The [d]HS3/Neo2- mutation reduces adult globin gene expression to 70% of the wild-type level.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory