Rr320^tm1Ley
Targeted Allele Detail

Summary

• Symbol: Rr320^tm1Ley
• Name: regulatory region 320; targeted mutation 1, Timothy Ley
• MGI ID: MGI:2662827
• Synonyms: Delta HS3/NEO +, Hbb-ar^tm1Ley
• Gene: Rr320 Location: Chr7:103501973-103526752 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: Rr320^tm1Ley page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33053
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A 2.3 kb genomic fragment encompassing the 5' DNase I hypersensitive site 3 (HS3) was replaced with a loxP site flanked neomycin resistance gene cassette inserted by homologous recombination. RT-PCR analysis indicated that the presence of neo cassette reduces Hbb expression by an additional 25 to 40% relative to Rr320^tm1.1Ley. (J:33053)

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr320 Mutation: 0 strains or lines available

References

• Original: J:33053 Hug BA, et al., Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3. Mol Cell Biol. 1996 Jun;16(6):2906-12
• All: 1 reference(s)