Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33053
Hug BA, et al., Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3. Mol Cell Biol. 1996 Jun;16(6):2906-12
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5484654
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Gene symbol:
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Hbb-b2
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Gene name:
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hemoglobin, beta adult minor chain
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Probe:
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Hbb-b-pA, Hbb-b-pB
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Probe preparation:
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DNA
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Visualized with:
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Phosphorimaging
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Assay notes:
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Restriction digestion of the RT-PCR products with BstXI cleaves the 129/SvJ product (178bp), but not the C57BL/6 product (200bp), as a result of a polymorphism between the two mouse strains. The digested samples were electrophoresed. Primers span intron 2 of both adult b-globin genes.
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Results
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Image: 2A 129/SvJ
| P adult
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TS28: blood
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Absent
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Not Specified; total RNA |
C57BL/6 |
| Not Specified |
| P adult
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TS28: blood
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Present
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Not Specified; total RNA |
129X1/SvJ |
| Not Specified |
| P adult
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TS28: blood
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Present
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Not Specified; total RNA |
(129/Sv x C57BL/6)F1 |
| Not Specified |
| P adult
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TS28: blood
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Weak
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Not Specified; total RNA |
involves: 129X1/SvJ * C57BL/6 |
Rr320tm1Ley/Rr320+ | Not Specified |
| P adult
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TS28: blood
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Present
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Not Specified; total RNA |
involves: 129X1/SvJ * C57BL/6 |
Rr320tm1.1Ley/Rr320+ | Not Specified |
Notes:
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Analysis Tools
