Antibody:	Anti-Nat5
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2A Nat5 , 2A Mdm20/Nat5 , 2A Mdm20/Nat5 low mag	Expression was detected in the dorsal telencephalon in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2B Mdm20/Nat5 , 2B Nat5 , 2B Mdm20/Nat5 low mag	Expression was detected in the ganglionic eminence in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2C Mdm20/Nat5 low mag , 2C Nat5 , 2C Mdm20/Nat5	Expression was detected in the preoptic area in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2D Mdm20/Nat5 low mag , 2D Nat5 , 2D Mdm20/Nat5	Expression was detected in the dorsal diencephalon in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2E Mdm20/Nat5 , 2E Mdm20/Nat5 low mag , 2E Nat5	Expression was detected in the posterior mesencephalon in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate	Present	Regionally restricted	2F Nat5 , 2F Mdm20/Nat5 , 2F Mdm20/Nat5 low mag	Expression was detected in the pons in a subset of PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	3A Nat5 , 3A Sox1/3 Nat5	Expression was detected in proliferating progenitors in the ventricular zone of the ganglionic eminence. Co-expression was noted with Sox1/3.

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Present	Regionally restricted	3B Nat5 , 3B Sox1/3 Nat5	Expression was detected in proliferating progenitors in the ventricular zone of the preoptic area. Co-expression was noted with Sox1/3.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Non-Uniform	3C Nat5 , 3C Sox1/3 Nat5	Expression was detected in proliferating progenitors in the ventricular zone of the ganglionic eminence. Co-expression was noted with Sox1/3.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Non-Uniform	5A Mdm20 Nat5 DAPI , 5A Nat5 , 5A Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Non-Uniform	5B Nat5 , 5B Mdm20 Nat5 , 5B Mdm20 Nat5 DAPI , 5B Mdm20 Nat5 Map2 , 5B Nat5 Map2	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Non-Uniform	5C Nat5 , 5C Mdm20 Nat5 DAPI , 5C Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present	Non-Uniform	5D Nat5 , 5D Mdm20 Nat5 DAPI , 5D Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Non-Uniform	5E Mdm20 Nat5 , 5E Mdm20 Nat5 DAPI , 5E Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: caudal ganglionic eminence	Present	Regionally restricted	5F Mdm20 Nat5 DAPI , 5F Nat5 , 5F Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: preoptic area	Present	Regionally restricted	5G Nat5 , 5G Mdm20 Nat5 , 5G Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	5H Nat5 Map2 , 5H Nat5 , 5H Mdm20 Nat5 , 5H Mdm20 Nat5 DAPI , 5H Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the bed nucleus stria terminalis and zona incerta. Co-expression with Mdm20 (Naa25) and Map2 was noted.
TS22: zona incerta	Present	Regionally restricted	5H Nat5 Map2 , 5H Nat5 , 5H Mdm20 Nat5 , 5H Mdm20 Nat5 DAPI , 5H Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the bed nucleus stria terminalis and zona incerta. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present	Non-Uniform	5I Mdm20 Nat5 , 5I Mdm20 Nat5 DAPI , 5I Nat5	Expression was detected in differentiating neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	6A Mdm20 Nat5 DAPI , 6A Mdm20 Nat5 , 6A Nat5	Expression was detected in differentiating neurons of the dorsal raphe of the mesencephalon. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6B Nat5 , 6B Mdm20 Nat5 DAPI , 6B Mdm20 Nat5	Expression was detected in differentiating neurons of the principal sensory trigeminal nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6C Mdm20 Nat5 DAPI , 6C Nat5 , 6C Mdm20 Nat5	Expression was detected in differentiating neurons of the vestibular nucleus. Expression was not detected in the facial nucleus. Co-expression with Mdm20 (Naa25) was noted in the vestibular nucleus.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6D Nat5 Map2 , 6D Mdm20 Nat5 DAPI , 6D Mdm20 Nat5 , 6D Nat5 , 6D Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the cochlear nucleus. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Absent		6E Mdm20 Nat5 , 6E Nat5 , 6E Mdm20 Nat5 DAPI	Expression was not detected in cerebellar deep neurons.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata basal plate	Present	Regionally restricted	6F Mdm20 Nat5 DAPI , 6F Nat5 Map2 , 6F Nat5 , 6F Mdm20 Nat5 , 6F Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the medullary raphe. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6G Nat5 , 6G Mdm20 Nat5 , 6G Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons of the spinal trigeminal nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6H Nat5 Map2 , 6H Mdm20 Nat5 Map2 , 6H Nat5 , 6H Mdm20 Nat5 DAPI , 6H Mdm20 Nat5	Expression was detected in differentiating neurons of the external cuneate nucleus. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Non-Uniform	7A , 7F	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.
TS26: lateral ventricle ependyma	Present	Non-Uniform	7A , 7B	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Present	Non-Uniform	7C , 7H	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: accumbens nucleus	Present	Non-Uniform	7D , 7I	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: zona incerta	Present	Regionally restricted	7J , 7E	Expression was detected in differentiated neurons in the zona incerta. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: pineal gland	Present	Non-Uniform	7G	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7O , 7K	Expression was detected in differentiated neurons in the cochlear nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7L , 7P	Expression was detected in differentiated neurons of the pontine reticular formation. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	7Q , 7M	Expression was detected in differentiated neurons of the external cuneate nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: inferior olivary nucleus	Present	Regionally restricted	7N , 7R	Expression was detected in differentiated neurons in the ventral portion of the inferior olivary nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Non-Uniform	7S Nat5 , 7S Merge	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Present	Non-Uniform	7T Merge , 7T Nat5	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: accumbens nucleus	Present	Non-Uniform	7U Nat5 , 7U Merge	Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7V Merge , 7V Nat5	Expression was detected in differentiated neurons in the cochlear nucleus. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7W Nat5 , 7W Merge	Expression was detected in differentiated neurons of the pontine reticular formation. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	7X Nat5 , 7X Merge	Expression was detected in differentiated neurons of the external cuneate nucleus. Co-expression with Mdm20 (Naa25) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: amygdala	Present	Non-Uniform		Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.
TS26: medullary raphe	Present	Non-Uniform		Expression was detected in differentiated neurons. Co-expression with Mdm20 (Naa25) was noted.
TS26: cerebral cortex	Present	Regionally restricted		Expression was detected in differentiated neurons in the cingulate cortex. Co-expression with Mdm20 (Naa25) was noted.
TS26: midbrain	Present	Regionally restricted		Expression was detected in differentiated neurons in the interpeduncular nucleus and the dorsal raphe. Co-expression with Mdm20 (Naa25) was noted.
TS26: medulla oblongata	Present	Regionally restricted		Expression was detected in differentiated neurons in the spinal trigeminal nucleus. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: preoptic area	Ambiguous		8A	Co-expression was not detected in preoptic area neurons.

Structure	Level	Pattern	Image	Note
TS26: pons	Ambiguous		8B	Co-expression was not detected in vestibular nucleus neurons.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Ambiguous		8C	Co-expression was not detected in thalamic paraventricular nucleus neurons.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Ambiguous		8D	Co-expression was not detected in lateral reticular nucleus neurons.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	9A	Expression was detected in dopaminergic neurons in the ventral tegmental area. Co-expression with Mdm20 (Naa25) was noted.
TS26: substantia nigra	Present	Regionally restricted	9A	Expression was detected in dopaminergic neurons in the substantia nigra. Co-expression with Mdm20 (Naa25) was noted.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	9B	Expression was detected in dopaminergic neurons in the ventral tegmental area. Co-expression with Mdm20 (Naa25) was noted.