Probe:	Mdm2 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present	Regionally restricted	1A	Expression was detected at the top of the neural folds containing neural crest progenitors.
TS11: embryo ectoderm	Weak	Regionally restricted	1A	Expression was fainter in the remainder of the epiblast.

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present	Regionally restricted	1B	Expression was detected at the top of the neural folds containing neural crest progenitors.
TS11: embryo ectoderm	Weak	Regionally restricted	1B	Expression was fainter in the remainder of the epiblast.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	1C	Expression was prominent in the dorsal part of the neural folds containg the pre-migratory neural crest cells.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	1D	Expression was prominent in the dorsal part of the neural folds containg the pre-migratory neural crest cells.
TS12: head mesenchyme derived from neural crest	Present	Regionally restricted	1D	Expression was detected in the frontonasal and periocular regions.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	1E	Expression was prominent in the dorsal part of the neural folds containg the pre-migratory neural crest cells.
TS12: migrating neural crest	Present	Regionally restricted	1E	Expression was detected in migrating cranial neural crest cells adjacent to the hindbrain region.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1F	Expression was prominent in the dorsal part of the neural folds containg the pre-migratory neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	1G	Expression was detected in the frontonasal and periocular regions.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	1H	Expression was detected in the frontonasal and periocular regions.
TS13: migrating neural crest	Present	Regionally restricted	1H	Expression was detected in migrating cranial neural crest cells streaming towards the first and future second branchial arches.
TS13: 1st branchial arch mesenchyme derived from neural crest	Present		1H

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	1I	Expression was detected in the frontonasal and periocular regions.
TS14: migrating neural crest	Present	Regionally restricted	1I	Expression was detected in migrating cranial neural crest cells streaming towards the first, future second and third branchial arches.
TS14: 1st branchial arch mesenchyme derived from neural crest	Present		1I

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	1J	Expression was detected in migrating cells from rhombomere 4.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	2A	Expression was detected in the dorsal part of the closing brain.
TS14: future midbrain	Present	Regionally restricted	2A	Expression was detected in the dorsal part of the closing brain.
TS14: migrating neural crest	Present	Regionally restricted	2A	Expression was detected in cells leaving the neural crest and in streams of cells migrating to the second branchial arch.
TS14: 1st branchial arch	Present		2A

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	2B	Expression was detected in the dorsal part of the closing brain.
TS14: prosencephalon	Present	Regionally restricted	2B	Expression was detected in the dorsal part of the closing brain.
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	2B	Expression was detected in cells leaving the neural crest and in the craniofacial mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	2C	Expression was detected in the craniofacial mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	2D	Expression was detected in the dorsal part of the closing brain.
TS14: future midbrain	Present	Regionally restricted	2D	Expression was detected in the dorsal part of the closing brain.
TS14: migrating neural crest	Present	Regionally restricted	2D	Expression was detected in cells leaving the neural crest and in subectodermal streams of cells migrating to the second branchial arch.
TS14: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2D	Expression was in subectodermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Present		2E	Expression corresponded with labeling observed with the neural crest-specific Crabp1 probe in an adjacent section.

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Regionally restricted	2G	Expression corresponded with labeling observed with the neural crest-specific Crabp1 probe in an adjacent section.