Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42028
Seitanidou T, et al., Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech Dev. 1997 Jul;65(1-2):31-42
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5436312
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Gene symbol:
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Egr2
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Gene name:
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early growth response 2
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1B:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more  )
Note: 2-4 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256).
Specimen
1B:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-4 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
1B
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Expression was detected in a narrow band of cells.
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Specimen
1C:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 2-4 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-4 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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Specimen
1E:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 4-6 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256).
Specimen
1E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-6 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
1E
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Expression was detected in prospective rhombomeres 3 and 5.
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Specimen
1F:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 4-6 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-6 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
1F
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Expression was detected in prospective rhombomeres 3 and 5.
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Specimen
1H:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 6-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
1H
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Expression was detected in prospective rhombomeres 3 and 5.
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Specimen
1I:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 6-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1I:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
1I
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Expression was detected in prospective rhombomeres 3 and 5.
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Specimen
1J:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 6-8 somites.Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1J:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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|
Note:
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6-8 somites.Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
1J
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Expression was detected in prospective rhombomeres 3 and 5.
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Specimen
1K:
embryonic day 8.5; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 10 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
1K:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
|
embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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Specimen
2A:
embryonic day 9.5; Egr2tm1Pch/Egr2 +
(more )
Note: 15-17 somites.
Specimen
2A:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
|
embryonic day 9.5
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|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15-17 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: head mesenchyme derived from neural crest |
Present |
Regionally restricted |
2A
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Expression was detected in neural crest cells emerging from the rhombomere 5/6 boundary region and migrating caudally to the otic vesicle.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
|
embryonic day 9.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15-17 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: head mesenchyme derived from neural crest |
Present |
Regionally restricted |
2D
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Expression was detected in neural crest cells emerging from rostral and caudal rhombomere 5 and migrating on both sides of the otic vesicle.
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Specimen
2E:
embryonic day 9.5; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 15-17 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
|
embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15-17 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple/blue - Epha4 (Sek-1; assay MGI:5436256)
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Specimen
4A:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 2 somites.
Specimen
4A:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
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|
Fixation:
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4% Paraformaldehyde
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|
Note:
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2 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4A
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Expression was detected in prospective rhombomere 3.
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Specimen
4C:
embryonic day 10.5; Egr2tm1Pch/Egr2 +
(more )
Note: Double labeled: turquoise/blue - Egr2 (Krox20); purple - Hoxb1 (assay MGI:5436406).
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
|
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Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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Double labeled: turquoise/blue - Egr2 (Krox20); purple - Hoxb1 (assay MGI:5436406).
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
|
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Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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Double labeled: turquoise/blue - Egr2 (Krox20); purple - Hoxb1 (assay MGI:5436406).
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Specimen
4E:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 7-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple - Fst (assay MGI:5436418).
Specimen
4E:
(close )
|
Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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7-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple - Fst (assay MGI:5436418).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4E
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Expression was detected in rhombomere 3 and 5.
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Specimen
4F:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 7-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple - Fst (assay MGI:5436418).
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
7-8 somites. Double labeled: turquoise/blue - Egr2 (Krox20); purple - Fst (assay MGI:5436418).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4F
|
Expression was detected in rhombomere 3 and 5.
|
Specimen
4I:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4I
|
Expression was detected in rhombomere 3 and 5. The expression in rhombomere 3 is slightly mosaic.
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Specimen
5A:
embryonic day 9.5; Egr2tm1Pch/Egr2 +
(more )
Note: Also stained for Msx1 (assay MGI:5436431).
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Also stained for Msx1 (assay MGI:5436431).
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Also stained for Msx1 (assay MGI:5436431).
|
Specimen
5C:
embryonic day 9.5; Egr2tm1Pch/Egr2 +
(more )
Note: Also stained for Wnt1 (assay MGI:5436433).
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Also stained for Wnt1 (assay MGI:5436433).
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Also stained for Wnt1 (assay MGI:5436433).
|
|
Analysis Tools